Connexin 43/GJA1 anticorps (AA 252-270)

N° du produit ABIN967717

Cet anticorps Souris Monoclonal détecte spécifiquement Connexin 43/GJA1 dans WB, IF, IP et IHC (f). Il présente une réactivité envers Humain, Rat, Souris, Chien et Poulet et a été mentionné dans 5+ publications.

Aperçu rapide pour Connexin 43/GJA1 anticorps (AA 252-270) (ABIN967717)

Antigène: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Reactivité: Humain, Rat, Souris, Chien, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Connexin 43/GJA1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 2-Connexin

Détail du produit anti-Connexin 43/GJA1 anticorps

Épitope: AA 252-270

Réactivité croisée: Poulet, Chien, Humain, Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat Connexin-43 aa. 252-270

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-Connexin 43/GJA1 (ABIN967717)

Giepmans, Hengeveld, Postma, Moolenaar: "Interaction of c-Src with gap junction protein connexin-43. Role in the regulation of cell-cell communication." dans: The Journal of biological chemistry, Vol. 276, Issue 11, pp. 8544-9, (2001) (PubMed).

Toyofuku, Akamatsu, Zhang, Kuzuya, Tada, Hori: "c-Src regulates the interaction between connexin-43 and ZO-1 in cardiac myocytes." dans: The Journal of biological chemistry, Vol. 276, Issue 3, pp. 1780-8, (2001) (PubMed).

Lucke, Choudhry, Thom, Selmer, Burden, Hodgins: "Upregulation of connexin 26 is a feature of keratinocyte differentiation in hyperproliferative epidermis, vaginal epithelium, and buccal epithelium." dans: The Journal of investigative dermatology, Vol. 112, Issue 3, pp. 354-61, (1999) (PubMed).

Laird, Castillo, Kasprzak: "Gap junction turnover, intracellular trafficking, and phosphorylation of connexin43 in brefeldin A-treated rat mammary tumor cells." dans: The Journal of cell biology, Vol. 131, Issue 5, pp. 1193-203, (1996) (PubMed).

Loo, Berestecky, Kanemitsu, Lau: "pp60src-mediated phosphorylation of connexin 43, a gap junction protein." dans: The Journal of biological chemistry, Vol. 270, Issue 21, pp. 12751-61, (1995) (PubMed).

Détails sur Connexin 43/GJA1

Antigène: Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))

Autre désignation: Connexin-43

Sujet: Gap junctions are intercellular protein pores. They enable cell-to-cell communication by allowing passage of ions and other small molecules. The subunits of gap junction channels are assembled from a family of proteins called connexins. Individual connexin molecules join to make hexameric hemichannels termed connexons, these structures dock to connexons on neighboring cells, forming gap junction pores. Connexin-43 is a member of the connexin family possessing four transmembrane regions, with cytoplasmic amino and carboxyl terminals. It undergoes rapid turnover in the cell and its monomers may reside in the ER/Golgi network, forming a reservoir available for assembly upon degradation of existing connexin-43 channels. In addition, it is believed that phosphorylation of connexin-43 plays a regulatory role both in the assembly of connexons and in gating activity at the gap junction. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 43 kDa

Pathways: Signalisation MAPK, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization