CDKN1B anticorps (AA 1-197)

N° du produit ABIN967850

L’anticorps Souris Monoclonal anti-CDKN1B a été validé pour WB, IHC, IF et IP. Il convient pour détecter CDKN1B dans des échantillons de Humain, Souris, Rat, Chien, Poulet et Grenouille. Il y a 7+ publications disponibles.

Aperçu rapide pour CDKN1B anticorps (AA 1-197) (ABIN967850)

Antigène: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Reactivité: Humain, Souris, Rat, Chien, Poulet, Grenouille

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CDKN1B est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 57-Kip1-p27

Détail du produit anti-CDKN1B anticorps

Épitope: AA 1-197

Réactivité croisée: Humain, Chien, Rat (Rattus), Poulet, Grenouille

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse Kip1 aa. 1-197

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-CDKN1B (ABIN967850)

Guo, Packard, Krolewski, Harris, Manglapus, Schwob: "Expression of pax6 and sox2 in adult olfactory epithelium." dans: The Journal of comparative neurology, Vol. 518, Issue 21, pp. 4395-418, (2011) (PubMed).

Martín-Ibáñez, Crespo, Urbán, Sergent-Tanguy, Herranz, Jaumot, Valiente, Long, Pineda, Andreu, Rubenstein, Marín, Georgopoulos, Mengod, Fariñas, Bachs, Alberch, Canals: "Ikaros-1 couples cell cycle arrest of late striatal precursors with neurogenesis of enkephalinergic neurons." dans: The Journal of comparative neurology, Vol. 518, Issue 3, pp. 329-51, (2010) (PubMed).

Poché, Furuta, Chaboissier, Schedl, Behringer: "Sox9 is expressed in mouse multipotent retinal progenitor cells and functions in Müller glial cell development." dans: The Journal of comparative neurology, Vol. 510, Issue 3, pp. 237-50, (2008) (PubMed).

Barnouin, Dubuisson, Child, Fernandez de Mattos, Glassford, Medema, Mann, Lam: "H2O2 induces a transient multi-phase cell cycle arrest in mouse fibroblasts through modulating cyclin D and p21Cip1 expression." dans: The Journal of biological chemistry, Vol. 277, Issue 16, pp. 13761-70, (2002) (PubMed).

Williamson, Dadmanesh, Koeffler: "BRCA1 transactivates the cyclin-dependent kinase inhibitor p27(Kip1)." dans: Oncogene, Vol. 21, Issue 20, pp. 3199-206, (2002) (PubMed).

Frey, Clark, Leontieva, Uronis, Black, Black: "Protein kinase C signaling mediates a program of cell cycle withdrawal in the intestinal epithelium." dans: The Journal of cell biology, Vol. 151, Issue 4, pp. 763-78, (2000) (PubMed).

Polyak, Kato, Solomon, Sherr, Massague, Roberts, Koff: "p27Kip1, a cyclin-Cdk inhibitor, links transforming growth factor-beta and contact inhibition to cell cycle arrest." dans: Genes & development, Vol. 8, Issue 1, pp. 9-22, (1994) (PubMed).

Détails sur CDKN1B

Antigène: CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))

Autre désignation: p27 Kip1

Sujet: Kip1 is a cyclin-dependent kinase (cdk) inhibitor that was identified as a result of its role in TGFbeta-induced G1 phase arrest and cell-cell contact. In vitro , p27 [Kip1] binds tightly to Cyclin D-Cdk4, Cyclin E-Cdk2, and Cyclin A-Cdk2 complexes and inhibits their activity. In normal cells, Kip1 is sequestered and its activity gradually decreases as cells reach S phase. The addition of TGF-beta in early G1 blocks this decline in activity, by preventing the sequestration of Kip1. TGF-beta also reduces the levels of Cdk4. Kip1 preferentially binds Cyclin D-Cdk4, but the lower levels of Cdk4 in TGF-beta treated cells allow Kip1 to be available for binding to Cyclin E-Cdk2 and Cyclin A-Cdk2. Kip1 is structurally related to Cip1/WAF1, having a similar 60 amino acid sequence in the N-terminal region. A 52 amino acid Kip1 peptide (residues 28-79) from this region is sufficient to inhibit cdk activity in vitro. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 27 kDa

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy