Cyclin D3 anticorps (AA 127-292)

N° du produit ABIN967879

Cet anticorps Souris Monoclonal détecte spécifiquement Cyclin D3 dans WB, IHC, IP et IF. Il présente une réactivité envers Humain, Souris, Rat et Chien et a été mentionné dans 5+ publications.

Aperçu rapide pour Cyclin D3 anticorps (AA 127-292) (ABIN967879)

Antigène: Cyclin D3 (CCND3)

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Cyclin D3 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 1-Cyclin D3

Détail du produit anti-Cyclin D3 anticorps

Épitope: AA 127-292

Réactivité croisée: Souris, Rat (Rattus), Chien

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Cyclin D3 aa. 127-292

Isotype: IgG2b

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-Cyclin D3 (ABIN967879)

Haller, Wu, Derow, Schmitt, Jeang, Grassmann: "Physical interaction of human T-cell leukemia virus type 1 Tax with cyclin-dependent kinase 4 stimulates the phosphorylation of retinoblastoma protein." dans: Molecular and cellular biology, Vol. 22, Issue 10, pp. 3327-38, (2002) (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." dans: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Bagui, Jackson, Agrawal, Pledger: "Analysis of cyclin D3-cdk4 complexes in fibroblasts expressing and lacking p27(kip1) and p21(cip1)." dans: Molecular and cellular biology, Vol. 20, Issue 23, pp. 8748-57, (2000) (PubMed).

Motokura, Keyomarsi, Kronenberg, Arnold: "Cloning and characterization of human cyclin D3, a cDNA closely related in sequence to the PRAD1/cyclin D1 proto-oncogene." dans: The Journal of biological chemistry, Vol. 267, Issue 28, pp. 20412-5, (1992) (PubMed).

Xiong, Menninger, Beach, Ward: "Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins." dans: Genomics, Vol. 13, Issue 3, pp. 575-84, (1992) (PubMed).

Détails sur Cyclin D3

Antigène: Cyclin D3 (CCND3)

Autre désignation: Cyclin D3

Sujet: Several classes of cyclins (A-E) have been described. These proteins act as regulatory subunits for cyclin-dependent kinases (cdks). The synthesis and degradation of cyclins is tightly controlled in a cell cycle specific manner. There are at least 3 different D-type cyclins whose relative levels vary among cell types. Cyclin D3 is known to associate with Cdk5 and weakly with Cdk2. Cdk4 is preferentially bound by Cyclin D1. However, in T cells which do not express Cyclin D1, Cdk4 associates with Cyclins D2 and D3. While bound to cdks, the D-type cyclins also associate with the polymerase-delta subunit, PCNA. Cyclin D3 has also been shown to bind pRb in vitro. This suggests that pRb may be an in vivo substrate of D-type cyclins. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 33 kDa

Pathways: Cycle Cellulaire, Mitotic G1-G1/S Phases, Glycosaminoglycan Metabolic Process