VASP anticorps (AA 248-379)

N° du produit ABIN967976

L’anticorps Souris Monoclonal anti-VASP a été validé pour WB, IHC, IF et IP. Il convient pour détecter VASP dans des échantillons de Humain et Chien. Il y a 5+ publications disponibles.

Aperçu rapide pour VASP anticorps (AA 248-379) (ABIN967976)

Antigène: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Reactivité: Humain, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp VASP est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 43-VASP

Détail du produit anti-VASP anticorps

Épitope: AA 248-379

Réactivité croisée: Chien

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human VASP aa. 248 - 379

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-VASP (ABIN967976)

DeMali, Barlow, Burridge: "Recruitment of the Arp2/3 complex to vinculin: coupling membrane protrusion to matrix adhesion." dans: The Journal of cell biology, Vol. 159, Issue 5, pp. 881-91, (2002) (PubMed).

Howe, Hogan, Juliano: "Regulation of vasodilator-stimulated phosphoprotein phosphorylation and interaction with Abl by protein kinase A and cell adhesion." dans: The Journal of biological chemistry, Vol. 277, Issue 41, pp. 38121-6, (2002) (PubMed).

Lawrence, Pryzwansky: "The vasodilator-stimulated phosphoprotein is regulated by cyclic GMP-dependent protein kinase during neutrophil spreading." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 9, pp. 5550-6, (2001) (PubMed).

Haffner, Jarchau, Reinhard, Hoppe, Lohmann, Walter: "Molecular cloning, structural analysis and functional expression of the proline-rich focal adhesion and microfilament-associated protein VASP." dans: The EMBO journal, Vol. 14, Issue 1, pp. 19-27, (1995) (PubMed).

Reinhard, Giehl, Abel, Haffner, Jarchau, Hoppe, Jockusch, Walter: "The proline-rich focal adhesion and microfilament protein VASP is a ligand for profilins." dans: The EMBO journal, Vol. 14, Issue 8, pp. 1583-9, (1995) (PubMed).

Détails sur VASP

Antigène: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Autre désignation: VASP

Sujet: Vasodilator-stimulated phosphoprotein (VASP), a substrate for cAMP- and cGMP-dependent kinases, is associated with actin filaments, focal adhesions, and dynamic membrane regions. VASP is composed of several distinct domains: a central L-proline-rich domain contains a GPPPPP motif as a single copy and as a three-fold tandem repeat, as well as three conserved phosphorylation sites for cyclic nucleotide-dependent protein kinases (Ser157, Ser239, and Thr278). A C-terminal domain contains a repetitive mixed-charge cluster which is predicted to form an alpha-helix. The C-terminal domain appears to be responsible for anchoring at focal adhesion sites. VASP has been shown to be a ligand for profilins. Profilins bind to the poly-L-proline motifs of VASP and it is postulated that these two molecules act in concert to convey signal transduction to actin filament formation.

Poids moléculaire: 46 kDa

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation