RAC1 anticorps

N° du produit ABIN968088

Cet anticorps anti-RAC1 Monoclonal Souris (Clone 102-Rac1) (ABIN968088) détecte spécifiquement RAC1 dans WB, IHC, IF et IP. L’anticorps est réactif avec des échantillons de Humain, Souris, Rat, Chien et Poulet.

Aperçu rapide pour RAC1 anticorps (ABIN968088)

Antigène: RAC1

Reactivité: Humain, Souris, Rat, Chien, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RAC1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 102-Rac1

Détail du produit anti-RAC1 anticorps

Réactivité croisée: Poulet, Chien, Souris, Rat (Rattus)

Attributs du produit: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
Positive Control: Rat Cerebrum

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG2b kappa

Information d'application

Indications d'application: WB: 1:1000

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Aqueous buffered solution containing BSA, glycerol.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Détails sur RAC1

Antigène: RAC1

Sujet: Rac1 is a member of the expanding family of Ras-related GTPase proteins. These small proteins have the ability to switch between two different conformational states: the active (GTP-bound) and the inactive (GDP-bound). The GTPase activity is increased by specific activator proteins named GAPs. Rac1, contains the consensus sequence Cys-X-X-X-COOH, which localizes it to the plasma membrane. Growth factor stimulation of cells results in a dramatic reorganization of the actin filament network and membrane ruffling, processes for which Rac1 is essential. Constitutive expression of Rac1 leads to cell transformation. This process is mediated by the Rac1-dependent activation of the p65PAK serine/threonine kinase. IQGAP1 has been identified as a GTPase activating protein specific for Rac1.
Testing in native ELISA with recombinant proteins to CDC42 and Rho have shown that the antibody to Rac1 (cat No ABIN968088/1) recognizes CDC42 and Rho. Due to sequence homology among various small GTPase familyl members, potential reactivity could be observed with other family members.

Poids moléculaire: 21 kDa

Pathways: Signalisation WNT, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling