RAC1 anticorps

N° du produit ABIN968089

Cet anticorps Souris Monoclonal détecte spécifiquement RAC1 dans WB, IHC, IF et IP. Il présente une réactivité envers Humain, Souris, Rat, Chien et Poulet et a été mentionné dans 5+ publications.

Aperçu rapide pour RAC1 anticorps (ABIN968089)

Antigène: RAC1

Reactivité: Humain, Souris, Rat, Chien, Poulet

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp RAC1 est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 102-Rac1

Détail du produit anti-RAC1 anticorps

Réactivité croisée: Rat (Rattus), Poulet, Chien, Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Rac1 Protein

Isotype: IgG2b kappa

Information d'application

Commentaires:

Related Products: ABIN967389, ABIN968545

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-RAC1 (ABIN968089)

Habas, Dawid, He: "Coactivation of Rac and Rho by Wnt/Frizzled signaling is required for vertebrate gastrulation." dans: Genes & development, Vol. 17, Issue 2, pp. 295-309, (2003) (PubMed).

Doye, Mettouchi, Bossis, Clément, Buisson-Touati, Flatau, Gagnoux, Piechaczyk, Boquet, Lemichez: "CNF1 exploits the ubiquitin-proteasome machinery to restrict Rho GTPase activation for bacterial host cell invasion." dans: Cell, Vol. 111, Issue 4, pp. 553-64, (2002) (PubMed).

Innocenti, Tenca, Frittoli, Faretta, Tocchetti, Di Fiore, Scita: "Mechanisms through which Sos-1 coordinates the activation of Ras and Rac." dans: The Journal of cell biology, Vol. 156, Issue 1, pp. 125-36, (2002) (PubMed).

Nobes, Hall: "Rho, rac, and cdc42 GTPases regulate the assembly of multimolecular focal complexes associated with actin stress fibers, lamellipodia, and filopodia." dans: Cell, Vol. 81, Issue 1, pp. 53-62, (1995) (PubMed).

Didsbury, Weber, Bokoch, Evans, Snyderman: "rac, a novel ras-related family of proteins that are botulinum toxin substrates." dans: The Journal of biological chemistry, Vol. 264, Issue 28, pp. 16378-82, (1989) (PubMed).

Détails sur RAC1

Antigène: RAC1

Sujet: Rac1 is a member of the expanding family of Ras-related GTPase proteins. These small proteins have the ability to switch between two different conformational states: the active (GTP-bound) and the inactive (GDP-bound). The GTPase activity is increased by specific activator proteins named GAPs. Rac1, like many of its relatives, contains the consensus sequence Cys-X-X-X-COOH, which localizes it to the plasma membrane. Growth factor stimulation of cells results in a dramatic reorganization of the actin filament network and membrane ruffling, processes for which Rac1 is essential. Constitutive expression of Rac1 leads to cell transformation. This process is mediated by the Rac1-dependent activation of the p65PAK serine/threonine kinase. In addition, IQGAP1 has been identified as a GTPase activating protein specific for Rac1. Due to sequence homology among various small GTPase family members, potential cross-reactivity could be observed with this antibody. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 21 kDa

Pathways: Signalisation WNT, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling