PCNA anticorps (AA 68-230)

N° du produit ABIN968097

Cet anticorps anti-PCNA est un anticorps Souris Monoclonal détectant PCNA dans WB, IHC, IF et IP. Adapté pour Humain, Souris, Rat et Chien. Ce Primary Antibody a été cité dans 5+ publications.

Aperçu rapide pour PCNA anticorps (AA 68-230) (ABIN968097)

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp PCNA est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 24-PC

Détail du produit anti-PCNA anticorps

Épitope: AA 68-230

Réactivité croisée: Souris, Rat (Rattus), Chien

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human PCNA aa. 68-230

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-PCNA (ABIN968097)

Li, Dowbenko, Lasky: "AKT/PKB phosphorylation of p21Cip/WAF1 enhances protein stability of p21Cip/WAF1 and promotes cell survival." dans: The Journal of biological chemistry, Vol. 277, Issue 13, pp. 11352-61, (2002) (PubMed).

Reilly, Wysocka, Herr: "Inactivation of the retinoblastoma protein family can bypass the HCF-1 defect in tsBN67 cell proliferation and cytokinesis." dans: Molecular and cellular biology, Vol. 22, Issue 19, pp. 6767-78, (2002) (PubMed).

Saitoh, Pizzi, Wang: "Perturbation of SUMOlation enzyme Ubc9 by distinct domain within nucleoporin RanBP2/Nup358." dans: The Journal of biological chemistry, Vol. 277, Issue 7, pp. 4755-63, (2002) (PubMed).

Moore, Scheinman, Bellgrau: "The identification of a novel T cell activation state controlled by a diabetogenic gene." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 166, Issue 1, pp. 241-8, (2001) (PubMed).

Travali, Ku, Rizzo, Ottavio, Baserga, Calabretta: "Structure of the human gene for the proliferating cell nuclear antigen." dans: The Journal of biological chemistry, Vol. 264, Issue 13, pp. 7466-72, (1989) (PubMed).

Détails sur PCNA

Antigène: PCNA (Proliferating Cell Nuclear Antigen (PCNA))

Autre désignation: PCNA

Sujet: Progression of the mammalian cell cycle is regulated in two different ways: 1) phosphorylation and dephosphorylation of key proteins, 2) synthesis and degradation of regulatory factors. The Proliferating Cell Nuclear Antigen (PCNA) was initially identified as a nuclear antigen in proliferating cells and was subsequently described as a subunit for DNA polymerase delta. Human PCNA is 262 amino acids with an apparent molecular weight of 36 kDa. PCNA protein levels peak during the S-phase of the cell cycle, at which time it forms a complex with the p21 inhibitor. PCNA is almost undetectable in other phases of the cycle. Because of its unique expression, PCNA has been extensively used in studies associating the prognosis of tumor progression and neoplastic proliferation.
Synonyms: Proliferating Cell Nuclear Antigen

Poids moléculaire: 36 kDa

Pathways: Telomere Maintenance, Réparation de l'ADN, Mitotic G1-G1/S Phases, DNA Replication, Synthesis of DNA, Autophagy