Dihydrofolate Reductase anticorps (AA 1-186)

N° du produit ABIN968119

Détail du produit anti-Dihydrofolate Reductase anticorps

Antigène: Dihydrofolate Reductase (DHFR)

Épitope: AA 1-186

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Dihydrofolate Reductase est non-conjugé

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Réactivité croisée: Souris, Humain, Chien, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Cow DHFR aa. 1-186

Clone: 49-DHFR

Isotype: IgG1

Information d'application

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-Dihydrofolate Reductase (ABIN968119)

Eymin, Gazzeri, Brambilla, Brambilla: "Distinct pattern of E2F1 expression in human lung tumours: E2F1 is upregulated in small cell lung carcinoma." dans: Oncogene, Vol. 20, Issue 14, pp. 1678-87, (2001) (PubMed).

de Wind, Dekker, Claij, Jansen, van Klink, Radman, Riggins, van der Valk, vant Wout, te Riele: "HNPCC-like cancer predisposition in mice through simultaneous loss of Msh3 and Msh6 mismatch-repair protein functions." dans: Nature genetics, Vol. 23, Issue 3, pp. 359-62, (1999) (PubMed).

Tang, Pao, Zhang: "Repair of benzo(a)pyrene diol epoxide- and UV-induced DNA damage in dihydrofolate reductase and adenine phosphoribosyltransferase genes of CHO cells." dans: The Journal of biological chemistry, Vol. 269, Issue 17, pp. 12749-54, (1994) (PubMed).

Israel, Kaufman: "Dexamethasone negatively regulates the activity of a chimeric dihydrofolate reductase/glucocorticoid receptor protein." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, Issue 9, pp. 4290-4, (1993) (PubMed).

Masters, Attardi: "The nucleotide sequence of the cDNA coding for the human dihydrofolic acid reductase." dans: Gene, Vol. 21, Issue 1-2, pp. 59-63, (1983) (PubMed).

Détails sur Dihydrofolate Reductase

Antigène: Dihydrofolate Reductase (DHFR)

Autre désignation: DHFR (DHFR Produits)

Synonymes: anticorps DHFR, anticorps F10_orf160, anticorps ECK0049, anticorps JW0047, anticorps tmrA, anticorps cb595, anticorps zgc:86637, anticorps DDBDRAFT_0187132, anticorps DDBDRAFT_0230113, anticorps DDB_0187132, anticorps DDB_0230113, anticorps CG14887, anticorps Dmel\\CG14887, anticorps dhfr, anticorps DHFRP1, anticorps DYR, anticorps 8430436I03Rik, anticorps AA607882, anticorps AI662710, anticorps AW555094, anticorps Dhfr1, anticorps confers resistance to sulfonamides, anticorps dihydrofolate reductase, anticorps dihydrofolate reductase DhfR, anticorps Dihydrofolate reductase, anticorps dhfr, anticorps DHFR, anticorps dhfR, anticorps folA, anticorps dfrA, anticorps LOC707382, anticorps CNJ01940, anticorps DDB_G0286755, anticorps Dhfr, anticorps Mesil_0096, anticorps Arch_0408, anticorps Fbal_3367, anticorps Ilyop_0482, anticorps Lbys_2007, anticorps Riean_0733, anticorps Mrub_1855, anticorps Poras_0766, anticorps Halhy_3552, anticorps Mesop_3614, anticorps LOC609048

Sujet: Dihydrofolate reductase (DHFR) regenerates tetrahydrofolate from dihydrofolate in the presence of NADPH. Tetrahydrofolate is the coenzyme for thymidilate synthetase in the biosynthesis of thymidine and is also critical for the synthesis of amino acids and purines. DHFR is a protein of 186 amino acids that is highly conserved among different organisms. DHFR levels change during the cell cycle, with the highest content during the G1/S transition. Expression of DHFR tightly correlates with the turnover rate of its mRNA. Because dividing cells require a continuous supply of thymidine, DHFR has been a target for anticancer drugs. The folic acid antagonists aminopterin and amethopterin (methotrexate), widely used in cancer treatments, inhibit DHFR. However, over long treatments, treated cells often amplify the DHFR gene, producing drug-resistant cells.

Poids moléculaire: 21 kDa

Pathways: Mitotic G1-G1/S Phases