ITGAL anticorps (AA 64-199)

N° du produit ABIN968200

Cet anticorps Souris Monoclonal détecte spécifiquement ITGAL dans WB, IP, IHC et IF. Il présente une réactivité envers Humain et a été mentionné dans 4+ publications.

Aperçu rapide pour ITGAL anticorps (AA 64-199) (ABIN968200)

Antigène: ITGAL (Integrin alpha-L (ITGAL))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ITGAL est non-conjugé

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunofluorescence (IF)

Clone: 27-CD11a

Détail du produit anti-ITGAL anticorps

Épitope: AA 64-199

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human LFA-1alpha aa. 64-199

Isotype: IgG1 kappa

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-ITGAL (ABIN968200)

Mamane, Grandvaux, Hernandez, Sharma, Innocente, Lee, Azimi, Lin, Hiscott: "Repression of IRF-4 target genes in human T cell leukemia virus-1 infection." dans: Oncogene, Vol. 21, Issue 44, pp. 6751-65, (2002) (PubMed).

Lub, van Kooyk, van Vliet, Figdor: "Dual role of the actin cytoskeleton in regulating cell adhesion mediated by the integrin lymphocyte function-associated molecule-1." dans: Molecular biology of the cell, Vol. 8, Issue 2, pp. 341-51, (1997) (PubMed).

Nueda, López-Cabrera, Vara, Corbí: "Characterization of the CD11a (alpha L, LFA-1 alpha) integrin gene promoter." dans: The Journal of biological chemistry, Vol. 268, Issue 26, pp. 19305-11, (1993) (PubMed).

Larson, Corbi, Berman, Springer: "Primary structure of the leukocyte function-associated molecule-1 alpha subunit: an integrin with an embedded domain defining a protein superfamily." dans: The Journal of cell biology, Vol. 108, Issue 2, pp. 703-12, (1989) (PubMed).

Détails sur ITGAL

Antigène: ITGAL (Integrin alpha-L (ITGAL))

Autre désignation: CD11a

Sujet: CD11a, also known as integrin alphaL or LFA-1 (lymphocyte function-associated antigen-1), is a member of the beta2 family of integrins that includes Mac-1 and p150,95. It is composed of two noncovalently linked alpha and beta chains which are associated with the actin cytoskeleton. CD11a (integrin alphaL) has been reported to be expressed on the surface of T cells, neutrophils, and macrophages. It is involved in cell-cell adhesion and it also conveys signals from the plasma membrane to the cytoplasm. Activation of the TCR/CD3 complex on T cells transmits intracellular signals that activate integrin alphaL (inside-out” signaling). This results in the conformational change of integrin alphaL, which increases its affinity for its ligand ICAM-1,-2, and -3. Furthermore, this signaling releases integrin alphaL from the actin cytoskeleton, resulting in clustering of this surface molecule. The high affinity conformation and the clustering of integrin alphaL increases the adhesion between it and ICAM. This adhesion is essential for leukocyte activation and results in outside-in” signaling which induces the polymerization of actin, thereby stabilizing the integrin alphaL-ICAM interaction. This interaction is necessary for CTL and NK mediated killing, T and B cell responses, ADCC, and immune cell extravasation. This antibody is routinely tested by western blot analysis.
Synonyms: Integrin alphaL, LFA-1alpha

Poids moléculaire: 180 kDa

Pathways: Activated T Cell Proliferation, Integrin Complex