ROCK1 anticorps (AA 906-1012)

N° du produit ABIN968386

Cet anticorps Souris Monoclonal détecte spécifiquement ROCK1 dans WB et IF. Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 5+ publications.

Aperçu rapide pour ROCK1 anticorps (AA 906-1012) (ABIN968386)

Antigène: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp ROCK1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 46-ROCK

Détail du produit anti-ROCK1 anticorps

Épitope: AA 906-1012

Réactivité croisée: Humain, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse ROCK-I aa. 906-1012

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968542, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-ROCK1 (ABIN968386)

Pawlak, Helfman: "Post-transcriptional down-regulation of ROCKI/Rho-kinase through an MEK-dependent pathway leads to cytoskeleton disruption in Ras-transformed fibroblasts." dans: Molecular biology of the cell, Vol. 13, Issue 1, pp. 336-47, (2002) (PubMed).

Wang, Eto, Steers, Somlyo, Somlyo: "RhoA-mediated Ca2+ sensitization in erectile function." dans: The Journal of biological chemistry, Vol. 277, Issue 34, pp. 30614-21, (2002) (PubMed).

Sahai, Olson, Marshall: "Cross-talk between Ras and Rho signalling pathways in transformation favours proliferation and increased motility." dans: The EMBO journal, Vol. 20, Issue 4, pp. 755-66, (2001) (PubMed).

Ishizaki, Maekawa, Fujisawa, Okawa, Iwamatsu, Fujita, Watanabe, Saito, Kakizuka, Morii, Narumiya: "The small GTP-binding protein Rho binds to and activates a 160 kDa Ser/Thr protein kinase homologous to myotonic dystrophy kinase." dans: The EMBO journal, Vol. 15, Issue 8, pp. 1885-93, (1996) (PubMed).

Nakagawa, Fujisawa, Ishizaki, Saito, Nakao, Narumiya: "ROCK-I and ROCK-II, two isoforms of Rho-associated coiled-coil forming protein serine/threonine kinase in mice." dans: FEBS letters, Vol. 392, Issue 2, pp. 189-93, (1996) (PubMed).

Détails sur ROCK1

Antigène: ROCK1 (rho-Associated, Coiled-Coil Containing Protein Kinase 1 (ROCK1))

Autre désignation: ROCK-I

Sujet: ROCK-I is a Rho-associated serine/threonine kinase isozyme that mediates RhoA-induced assembly of focal adhesions and actin stress fibers. It contains an N-terminal kinase domain, a central 600 amino acid long coiled-coil region, a C-terminal pleckstrin homology region (PH) and a Cys-rich zinc finger motif. The ROCK-I kinase domain is approximately 90% identical to that of ROCK-II. ROCK-I binds GTP-bound Rho through a Rho-binding domain (RBD). As a result, the kinase activity of ROCK-I is moderately stimulated. The ROCK isozymes regulate cell contractility through phosphorylation of the myosin light chain. This effect results from either the inhibition of the myosin phosphatase or by direct phosphorylation of the myosin light chain, thus bypassing the myosin light chain kinase. In addition, ROCK-I activates the ubiquitously expressed Na-H exchanger (NHE1) via a number of mechanisms including RhoA. NHE1 may mediate ROCK-I-induced changes in the actin cytoskeleton. Therefore, ROCK-I plays an important role in the regulation of focal adhesion and stress fiber formation.
Synonyms: ROKbeta

Poids moléculaire: 160 kDa

Pathways: Dynamique des Microtubules, Signalisation WNT, M Phase, Maintenance of Protein Location, Signaling Events mediated by VEGFR1 and VEGFR2, Thromboxane A2 Receptor Signaling