IRS1 anticorps (AA 1131-1234)

N° du produit ABIN968515

Cet anticorps Souris Monoclonal détecte spécifiquement IRS1 dans WB et IF. Il présente une réactivité envers Souris et Rat et a été mentionné dans 5+ publications.

Aperçu rapide pour IRS1 anticorps (AA 1131-1234) (ABIN968515)

Antigène: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Reactivité: Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp IRS1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 6-IRS

Détail du produit anti-IRS1 anticorps

Épitope: AA 1131-1234

Réactivité croisée: Souris

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat IRS-1 aa. 1131-1234

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-IRS1 (ABIN968515)

Obici, Feng, Karkanias, Baskin, Rossetti: "Decreasing hypothalamic insulin receptors causes hyperphagia and insulin resistance in rats." dans: Nature neuroscience, Vol. 5, Issue 6, pp. 566-72, (2002) (PubMed).

Paz, Liu, Shorer, Hemi, LeRoith, Quan, Kanety, Seger, Zick: "Phosphorylation of insulin receptor substrate-1 (IRS-1) by protein kinase B positively regulates IRS-1 function." dans: The Journal of biological chemistry, Vol. 274, Issue 40, pp. 28816-22, (1999) (PubMed).

Kuhné, Zhao, Rowles, Lavan, Shen, Fischer, Lienhard: "Dephosphorylation of insulin receptor substrate 1 by the tyrosine phosphatase PTP2C." dans: The Journal of biological chemistry, Vol. 269, Issue 22, pp. 15833-7, (1994) (PubMed).

Backer, Myers, Shoelson, Chin, Sun, Miralpeix, Hu, Margolis, Skolnik, Schlessinger: "Phosphatidylinositol 3'-kinase is activated by association with IRS-1 during insulin stimulation." dans: The EMBO journal, Vol. 11, Issue 9, pp. 3469-79, (1992) (PubMed).

Sun, Rothenberg, Kahn, Backer, Araki, Wilden, Cahill, Goldstein, White: "Structure of the insulin receptor substrate IRS-1 defines a unique signal transduction protein." dans: Nature, Vol. 352, Issue 6330, pp. 73-7, (1991) (PubMed).

Détails sur IRS1

Antigène: IRS1 (Insulin Receptor Substrate 1 (IRS1))

Autre désignation: IRS-1

Sujet: The IRS (Insulin receptor substrate) proteins IRS-1, IRS-2, IRS-3, and IRS-4 are major substrates of the insulin receptor tyrosine kinase and the insulin-like growth factor-1 receptor. IRS proteins contain an N-terminal pleckstrin homology (PH) domain, an ATP-binding domain, and multiple tyrosine phosphorylation sites in the C-terminus. Following insulin receptor ligation, IRS-1 binds to the juxtamembrane region of the receptor and is tyrosine phosphorylated. This facilitates its interaction with SH2 domain-containing signaling proteins, such as PI3 kinase, fyn, Grb2, and PTP1D. Phosphorylation dramatically reduces the affinity of IRS-1 for the insulin receptor, indicating that dissociation from the receptor and subsequent subcellular translocation are important to IRS-1 function in the pleiotropic effects induced by insulin. In support of this, IRS-1-null mice are viable, but exhibit growth retardation and abnormal glucose metabolism. In cases of reduced IRS-1 expression, certain IRS-1 functions can be assumed by the related IRS-2 protein, while other activities linked to IRS-1 are inhibited. Thus, IRS-1 is an essential component of insulin induced signal transduction. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 180 kDa

Pathways: Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Hormone Transport, Negative Regulation of Hormone Secretion, Response to Growth Hormone Stimulus, Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process