TOPBP1 anticorps (AA 204-416)

N° du produit ABIN968690

L’anticorps Souris Monoclonal anti-TOPBP1 a été validé pour WB et IF. Il convient pour détecter TOPBP1 dans des échantillons de Humain. Il y a 3+ publications disponibles.

Aperçu rapide pour TOPBP1 anticorps (AA 204-416) (ABIN968690)

Antigène: TOPBP1 (Topoisomerase (DNA) II Binding Protein 1 (TOPBP1))

Reactivité: Humain

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp TOPBP1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 33-TopBP1

Détail du produit anti-TOPBP1 anticorps

Épitope: AA 204-416

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human TopBP1 aa. 204-416

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN968537, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-TOPBP1 (ABIN968690)

Yamane, Katayama, Tsuruo: "The BRCT regions of tumor suppressor BRCA1 and of XRCC1 show DNA end binding activity with a multimerizing feature." dans: Biochemical and biophysical research communications, Vol. 279, Issue 2, pp. 678-84, (2001) (PubMed).

Yamane, Tsuruo: "Conserved BRCT regions of TopBP1 and of the tumor suppressor BRCA1 bind strand breaks and termini of DNA." dans: Oncogene, Vol. 18, Issue 37, pp. 5194-203, (1999) (PubMed).

Yamane, Kawabata, Tsuruo: "A DNA-topoisomerase-II-binding protein with eight repeating regions similar to DNA-repair enzymes and to a cell-cycle regulator." dans: European journal of biochemistry / FEBS, Vol. 250, Issue 3, pp. 794-9, (1998) (PubMed).

Détails sur TOPBP1

Antigène: TOPBP1 (Topoisomerase (DNA) II Binding Protein 1 (TOPBP1))

Autre désignation: TopBP1

Sujet: Eukaryotic DNA topoisomerase II, a ubiquitous ATP-dependent type II topoisomerase, is an essential nuclear enzyme in DNA replication and transcription, chromatin segregation, and cell cycle progression. Topoisomerases transiently break a pair of complementary strands in double-stranded DNA to form a gate for the passage of duplex DNA. Two isoforms of DNA topoisomerase II have been identified: topo IIalpha and topo IIbeta. These exhibit a high degree of homology, except for some divergence in the C-terminal region. Both contain multiple bipartite nuclear localization sequences (NLS) that mediate their subnuclear localization. DNA Topoisomerase II Binding Protein 1 (TopBP1) binds the C-terminal region of Topo IIbeta via its N-terminal region. In addition, TopBP1 contains a putative ADP-ribosylation site, two N-terminal NLS domains, and 8 repeating BRCA1 C-terminal (BRCT) domains found in DNA repair proteins, such as BRCA1, XRCC1, and Rad4. The BRCT domains of TopBP1 have been shown to bind DNA breaks, DNA nicks, and DNA termini, but not circular intact DNA. Thus, TopBP1 may localize topoisomerases to sites of DNA damage.
Synonyms: Topoisomerase II Binding Protein 1

Poids moléculaire: 161 kDa

Pathways: Chromatin Binding