Calreticulin anticorps (AA 270-390)

N° du produit ABIN968752

Cet anticorps Souris Monoclonal détecte spécifiquement Calreticulin dans WB et IF. Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 4+ publications.

Aperçu rapide pour Calreticulin anticorps (AA 270-390) (ABIN968752)

Antigène: Calreticulin (CALR)

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Calreticulin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 16-Calreticulin

Détail du produit anti-Calreticulin anticorps

Épitope: AA 270-390

Réactivité croisée: Humain, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Mouse Calreticulin aa. 270-390

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-Calreticulin (ABIN968752)

Ito, Seyama, Kubota: "Calreticulin is directly involved in anti-alpha3 integrin antibody-mediated secretion and activation of matrix metalloprotease-2." dans: Biochemical and biophysical research communications, Vol. 283, Issue 2, pp. 297-302, (2001) (PubMed).

Saito, Ihara, Leach, Cohen-Doyle, Williams: "Calreticulin functions in vitro as a molecular chaperone for both glycosylated and non-glycosylated proteins." dans: The EMBO journal, Vol. 18, Issue 23, pp. 6718-29, (2000) (PubMed).

Nair, Wearsch, Mitchell, Wassenberg, Gilboa, Nicchitta: "Calreticulin displays in vivo peptide-binding activity and can elicit CTL responses against bound peptides." dans: Journal of immunology (Baltimore, Md. : 1950), Vol. 162, Issue 11, pp. 6426-32, (1999) (PubMed).

Mazzarella, Gold, Cunningham, Green: "Determination of the sequence of an expressible cDNA clone encoding ERp60/calregulin by the use of a novel nested set method." dans: Gene, Vol. 120, Issue 2, pp. 217-25, (1992) (PubMed).

Détails sur Calreticulin

Antigène: Calreticulin (CALR)

Autre désignation: Calreticulin

Sujet: Proteins undergo numerous modifications, including folding, translocation, and degradation. During such modifications, polypeptides are rarely in a native, stable state. Molecular chaperones are a diverse group of proteins that modulate polypeptide stability through ATP-dependent folding. Many chaperone proteins are found in the endoplasmic reticulum (ER). Calreticulin is a luminal ER protein that is 39% homologous to the ER chaperone protein, calnexin. Calreticulin contains a C-terminal KDEL ER retention signal, and can bind Ca2+, Zn2+, ATP, and the proteins, ERp57 and protein disulfide isomerase. The molecular chaperone activities of calreticulin may include folding of both Asn-linked glycoproteins and non-glycosylated proteins. In addition, calreticulin is a component of MHC I/transporter associated with Ag presentation (TAP) complex where it may function in peptide assembly onto nascent class I molecules. Calreticulin may also function in integrin signaling, since it binds a3-integrin subunits and regulates integrin-mediated metalloprotease secretion. Thus, calreticulin may be involved in Ca2+ storage, cell adhesion, and protein folding. This antibody is routinely tested by western blot analysis.

Poids moléculaire: 60 kDa

Pathways: Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, ER-Nucleus Signaling, Unfolded Protein Response