Nicastrin anticorps (AA 168-289)

N° du produit ABIN968805

Cet anticorps anti-Nicastrin est un anticorps Souris Monoclonal détectant Nicastrin dans WB et IF. Adapté pour Humain, Souris, Rat, Poulet et Chien. Ce Primary Antibody a été cité dans 2+ publications.

Aperçu rapide pour Nicastrin anticorps (AA 168-289) (ABIN968805)

Antigène: Nicastrin (NCSTN)

Reactivité: Humain, Souris, Rat, Poulet, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Nicastrin est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 35-Nicastrin

Détail du produit anti-Nicastrin anticorps

Épitope: AA 168-289

Réactivité croisée: Poulet, Chien, Souris, Rat (Rattus)

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Human Nicastrin aa. 168-289

Isotype: IgG2a

Information d'application

Commentaires:

Related Products: ABIN967389, ABIN968553

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-Nicastrin (ABIN968805)

Chen, Yu, Arawaka, Nishimura, Kawarai, Yu, Tandon, Supala, Song, Rogaeva, Milman, Sato, Yu, Janus, Lee, Song, Zhang, Fraser, St George-Hyslop: "Nicastrin binds to membrane-tethered Notch." dans: Nature cell biology, Vol. 3, Issue 8, pp. 751-4, (2001) (PubMed).

Yu, Nishimura, Arawaka, Levitan, Zhang, Tandon, Song, Rogaeva, Chen, Kawarai, Supala, Levesque, Yu, Yang, Holmes, Milman, Liang, Zhang, Xu, Sato, Rogaev, Smith, Janus, Zhang, Aebersold, Farrer, Sorbi et al.: "Nicastrin modulates presenilin-mediated notch/glp-1 signal transduction and betaAPP processing. ..." dans: Nature, Vol. 407, Issue 6800, pp. 48-54, (2000) (PubMed).

Détails sur Nicastrin

Antigène: Nicastrin (NCSTN)

Autre désignation: Nicastrin

Sujet: Amyloid precursor protein (APP) gene encodes multiple APPs ranging from 695 to 770 amino acids. The unprocessed form of APP is a putative cell surface receptor that possesses neurite-promoting activity, and is involved in synaptic vesicle recycling. Processing of APP by sequential enzymatic activity of beta- and gamma-secretase, and the presenilin proteins PS1 and PS2, produces APP fragments that may have unique functions. beta-secretase activity cleaves the extracellular portion of APP leading to asecreted APP form, while gamma-secretase activity produces beta-Amyloid peptide (39-43 amino acids). The Abeta peptide produces abnormal plaques in the cerebral cortex and blood vesselwalls during Alzheimer's disease. Nicastrin is a PS1 associated transmembrane protein,that contains N-terminal glycosylation and myristoylation sites. Nicastrin can bind full length APP and the fragments produced by gamma-secretase. In C. elegans, suppression of nicastrin expression produces phenotypes that mimic those produced when notch signaling proteins are suppressed. In Drosophila, deficiencies in nicastrin prevent cleavage of the intracellular portion of Notch. Thus, nicastrin may be a functional component of presenilins and gamma-secretase complexes, which process Notch and APP transmembrane receptors.

Poids moléculaire: 110 kDa

Pathways: Signalisation Notch, Neurotrophin Signaling Pathway