CDK1 anticorps (pTyr15)

N° du produit ABIN968812

Cet anticorps Souris Monoclonal détecte spécifiquement CDK1 dans WB et IF. Il présente une réactivité envers Humain, Souris et Rat et a été mentionné dans 2+ publications.

Aperçu rapide pour CDK1 anticorps (pTyr15) (ABIN968812)

Antigène: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Reactivité: Humain, Souris, Rat

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp CDK1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 44-Cdk1-Cdc2

Détail du produit anti-CDK1 anticorps

Épitope: pTyr15

Réactivité croisée (Details): Predicted: Mouse, Rat

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Phosphorylated Human Cdc2 (pY15) Peptide

Isotype: IgG1

Information d'application

Commentaires:

Related Products: ABIN967389, ABIN967704

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20°C.

Références pour anticorps anti-CDK1 (ABIN968812)

Borgne, Meijer: "Sequential dephosphorylation of p34(cdc2) on Thr-14 and Tyr-15 at the prophase/metaphase transition." dans: The Journal of biological chemistry, Vol. 271, Issue 44, pp. 27847-54, (1996) (PubMed).

Uckun, Tuel-Ahlgren, Waddick, Jun, Jin, Myers, Rowley, Burkhardt, Bolen: "Physical and functional interactions between Lyn and p34cdc2 kinases in irradiated human B-cell precursors." dans: The Journal of biological chemistry, Vol. 271, Issue 11, pp. 6389-97, (1996) (PubMed).

Détails sur CDK1

Antigène: CDK1 (Cyclin-Dependent Kinase 1 (CDK1))

Autre désignation: Cdk1/Cdc2

Sujet: Progression of the mammalian cell cycle is regulated by phosphorylation of many key proteins. Several classes of cyclins (A-E) act as regulatory subunits for cyclin-dependent kinases (cdks). Cdk1/Cdc2 (p34 [cdc2]) is the catalytic subunit of the maturation promoting factor (MPF), which includes the regulatory subunit cyclin B. During late S and G2 phase, cyclin B synthesis increases, allowing it to bind Cdc2. This begins the transition into M-phase of the mammalian cell cycle by initiating a series of phosphorylation and dephosphorylation events that lead to activation of the Cdc2/cyclin B complex. After binding to cyclin B, cdc2 is phosphorylated on Thr-14, by Myt1, and Tyr-15, by wee1 or mik1, yielding an inactive pre-MPF complex during G2 phase. Phosphorylation of cdc2 on Thr-161 is performed by a cdk7/cyclin H complex and is necessary for activation of the cdc2 complex. Dephosphorylation of Thr-14 and Tyr-15 by CDC25 occurs at the end of G2 phase and completes activation of the cdc2/Cyclin B complex and facilitates entry into mitosis. During mitosis, cyclin B is targeted for degradation and Cdc2 becomes inactive again.
Synonyms: p34 [cdc2]

Poids moléculaire: 34 kDa

Pathways: Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA