Dynactin 1 anticorps (AA 3-202)

N° du produit ABIN968918

L’anticorps Souris Monoclonal anti-Dynactin 1 a été validé pour WB, IF et BI. Il convient pour détecter Dynactin 1 dans des échantillons de Humain, Souris, Rat et Chien. Il y a 2+ publications disponibles.

Aperçu rapide pour Dynactin 1 anticorps (AA 3-202) (ABIN968918)

Antigène: Dynactin 1 (DCTN1)

Reactivité: Humain, Souris, Rat, Chien

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp Dynactin 1 est non-conjugé

Application: Western Blotting (WB), Immunofluorescence (IF), BioImaging (BI)

Clone: 12-P150GLUED

Détail du produit anti-Dynactin 1 anticorps

Épitope: AA 3-202

Réactivité croisée: Souris, Humain, Chien

Attributs du produit: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogène: Rat p150 [Glued] aa. 3-202

Isotype: IgG1

Information d'application

Indications d'application: Methanol Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 90% methanol. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Wash three times with PBS. Flick out PBS and add second step reagent. Incubate for 1 hour at RT. Wash three times with PBS.

Triton-X 100 Procedure for a 96 well plate: Remove media from wells. Add 100 µl/well fresh 3.7% Formaldehyde in PBS. Incubate for 10 minutes at room temperature (RT). Flick out and add 100 µl/well 0.1% Triton-X 100. Incubate for 5 minutes at RT. Flick out and wash twice with PBS. Flick out PBS and add 100 µl/well blocking buffer (3% FBS in PBS). Incubate for 30 minutes at RT. Flick out and add diluted antibody (diluted in blocking buffer). Incubate for 1 hour at RT. Flick out and wash three times with PBS. Flick out and add second step reagent. Incubate for 1 hour at RT. Flick out and wash three times with PBS.

Commentaires:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: -20 °C

Stockage commentaire: Store undiluted at -20° C.

Références pour anticorps anti-Dynactin 1 (ABIN968918)

Waterman-Storer, Karki, Holzbaur: "The p150Glued component of the dynactin complex binds to both microtubules and the actin-related protein centractin (Arp-1)." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 5, pp. 1634-8, (1995) (PubMed).

Holzbaur, Hammarback, Paschal, Kravit, Pfister, Vallee: "Homology of a 150K cytoplasmic dynein-associated polypeptide with the Drosophila gene Glued." dans: Nature, Vol. 351, Issue 6327, pp. 579-83, (1991) (PubMed).

Détails sur Dynactin 1

Antigène: Dynactin 1 (DCTN1)

Autre désignation: p150 Glued

Sujet: P150 [Glued] was identified as a polypeptide associated with cytoplasmic dynein,the minus-end-directed microtubule-based motor protein. p150 [Glued] is also a member of the oligomeric dynactin complex. Dynactin mediates dynein-driven vesicle motility, as well as lower eukaryote nuclear transport. p150 [Glued] bears significant homology to the product of the Glued gene in Drosophila. It has been shown in vitro to be a required activator of dynein-mediated transport along microtubules. The p150 [Glued] component of the dynactin complex binds to microtubules and the actin-like protein Centractin (Arp-1), another member of the dynactin complex. In the developing rat, p150 [Glued] is expressed at high levels in neural tissue. Microtubule binding assays with selected constructs of p150 [Glued] indicate that amino acids 39-150 are required for microtubule association.

Poids moléculaire: 150 kDa

Pathways: M Phase, ER-Nucleus Signaling