E2F1 anticorps (AA 69-223)

N° du produit ABIN969516

Cet anticorps anti-E2F1 est un anticorps Souris Monoclonal détectant E2F1 dans WB, ELISA, IHC et FACS. Adapté pour Humain et Souris. Ce Primary Antibody a été cité dans 4+ publications.

Aperçu rapide pour E2F1 anticorps (AA 69-223) (ABIN969516)

Antigène: E2F1 (E2F Transcription Factor 1 (E2F1))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp E2F1 est non-conjugé

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 8G9

Détail du produit anti-E2F1 anticorps

Épitope: AA 69-223

Fonction: E2F1 Antibody

Purification: Purified antibody

Immunogène: Purified recombinant fragment of human E2F1 (AA: 69-223) expressed in E. Coli.

Isotype: IgG1

Information d'application

Indications d'application:

ELISA: 1/10000

FCM: 1/200 - 1/400

Restrictions: For Research Use only

Stockage

Format: Liquid

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Références pour anticorps anti-E2F1 (ABIN969516)

Zhou, Jiang, Xia, Zhang: "Comparison of the therapeutic effects of lobaplatin and carboplatin on retinoblastoma in vitro and in vivo." dans: International journal of oncology, Vol. 57, Issue 3, pp. 697-706, (2020) (PubMed).

Okazaki, Matsunaga, Okazaki, Utoguchi, Suzuki, Maruyama, Koyanagi, Ohdo: "Circadian rhythm of transferrin receptor 1 gene expression controlled by c-Myc in colon cancer-bearing mice." dans: Cancer research, Vol. 70, Issue 15, pp. 6238-46, (2010) (PubMed).

Gurtner, Fuschi, Martelli, Manni, Artuso, Simonte, Ambrosino, Antonini, Folgiero, Falcioni, Sacchi, Piaggio: "Transcription factor NF-Y induces apoptosis in cells expressing wild-type p53 through E2F1 upregulation and p53 activation." dans: Cancer research, Vol. 70, Issue 23, pp. 9711-20, (2010) (PubMed).

Locatelli, Bosotti, Ciomei, Brasca, Calogero, Mercurio, Fiorentini, Bertolotti, Scacheri, Scaburri, Galvani, Pesenti, De Baere, Soria, Lazar, Isacchi: "Transcriptional analysis of an E2F gene signature as a biomarker of activity of the cyclin-dependent kinase inhibitor PHA-793887 in tumor and skin biopsies from a phase I clinical study." dans: Molecular cancer therapeutics, Vol. 9, Issue 5, pp. 1265-73, (2010) (PubMed).

Détails sur E2F1

Antigène: E2F1 (E2F Transcription Factor 1 (E2F1))

Autre désignation: E2F1

Sujet: The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.

Poids moléculaire: 46.9 kDa

ID gène: 1869

UniProt: Q01094

Pathways: Signalisation p53, Cycle Cellulaire, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Autophagy