HDAC1 anticorps (AA 385-396)

N° du produit ABIN987284

L’anticorps Chèvre Polyclonal anti-HDAC1 a été validé pour WB, ELISA et ChIP. Il convient pour détecter HDAC1 dans des échantillons de Humain et Souris.

Aperçu rapide pour HDAC1 anticorps (AA 385-396) (ABIN987284)

Antigène: HDAC1 (Histone Deacetylase 1 (HDAC1))

Reactivité: Humain, Souris

Hôte: Chèvre

Clonalité: Polyclonal

Conjugué: Cet anticorp HDAC1 est non-conjugé

Application: Western Blotting (WB), ELISA, Chromatin Immunoprecipitation (ChIP)

Classe de qualité: Verified

Détail du produit anti-HDAC1 anticorps

Épitope: AA 385-396

Fonction: Hdac1 (aa385-396)

Séquence: PEDAIPEESG DE

Specificité: This antibody may cross-react to HDAC2

Réactivité croisée: Boeuf (Vache), Humain, Souris, Porc, Rat

Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Immunogène: Peptide with sequence C-PEDAIPEESGDE, from the internal region of the protein sequence according to NP_032254.1.

Isotype: IgG

Information d'application

Indications d'application:

Western Blot: Approx 55 kDa band observed in lysates of cell line NIH3T3 (calculated MW of 55.1 kDa according to Human NP_032254.1 and Mouse NP_032254.1). Recommended concentration: 0.3-1 μg/mL.

Peptide ELISA: antibody detection limit dilution 1:1000.

Commentaires:

Chromatin Immunoprecipitation: A 4-fold increased presence of H3 onto the Human P21 locus observed when comparing the precipitations using EB11428 with non-specific goat IgG to bring down HDAC1 in a chromatin lysate from MCF7 cells.

Restrictions: For Research Use only

Stockage

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Conseil sur la manipulation: Minimize freezing and thawing.

Stock: -20 °C

Stockage commentaire: Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.

Détails sur HDAC1

Antigène: HDAC1 (Histone Deacetylase 1 (HDAC1))

Autre désignation: Hdac1

Sujet: HDAC1, histone deacetylase 1, RP23-209C6.7, HD1, Hdac1-ps, MommeD5, RPD3, MGC102534, MGC118085, OTTMUSP00000010171

ID gène: 3065, 433759, 84576

NCBI Accession: NP_032254

Pathways: Neurotrophin Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Mitotic G1-G1/S Phases, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Negative Regulation of intrinsic apoptotic Signaling, Embryonic Body Morphogenesis