TGFB1 Kit ELISA

N° du produit ABIN1112676

Ce kit ELISA Souris TGFB1 est un kit ELISA Colorimetric conçu pour quantifier Souris TGFB1.

Aperçu rapide pour TGFB1 Kit ELISA (ABIN1112676)

Antigène: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Tissue Lysate

Détail du produit TGFB1 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Fonction: For quantitative detection of TGF-1 in mouse serum, body fluids, tissue lysates or cell culture supernatants.

Analytical Method: Quantitative

Sensibilité: < 2 pg/mL

Ingrédients: 1. One 96-well plate pre-coated with anti-Mouse TGFbeta1 antibody 2. Lyophilized Mouse TGFbeta1 standards: 2 tubes (10ng / tube) 3. Sample / Standard diluent buffer: 30ml 4. Biotin conjugated anti-Mouse TGFbeta1 antibody (Concentrated): 130 µl.

Matériel non inclus: 1. 37 °C incubator 2. Microplate reader (wavelength: 450nm) 3. Precise pipette and disposable pipette tips 4. Automated plate washer 5. ELISA shaker 6. 1.5ml of Eppendorf tubes 7. Plate cover 8. Absorbent filter papers 9. Plastic or glass container with volume of above 1L

Information d'application

Commentaires:

This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TGF?1 polyclonal antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TGF?1 polyclonal antibody was used as detection antibodies. The standards test samples and biotin conjugated detection antibody were added - the wells subsequently and wash with wash buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with wash buffer. TMB substrates were used - visualize HRP enzymatic reaction. TMB was catalyzed by HRP - produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional - the TGF?1 amount of sample captured in plate. Read the O.D. absorbance at 450 nm in a microplate reader and then the concentration of TGF?1 can be calculated.

Plaque: Pre-coated

Préparation des réactifs:

1. Before the experiment, centrifuge each kit component for several minutes to bring down all reagents to the bottom of tubes. 2. It is recommend to measure each standard and sample in duplicate. 3. Do NOT let the plate completely dry at any time! Since the dry condition can inactivate the biological material on the plate. 4. Do not reuse pipette tips and tubes to avoid cross contamination. 5. Do not use the expired components and the components from different batches. 6. To avoid the marginal effect of plate incubation for temperature differences (the marginal wells always get stronger reaction), it is recommend to equilibrate the ABC working solution and TMB substrate for at least 30 min at room temperature (37°C ) before adding to wells.The TMB substrate (Kit Component 8) is colorless and transparent before use, if not, please contact us for replacement.

Préparation de l'échantillon:

Preparation of sample and reagents 1. Sample Isolate the test samples soon after collecting, then, analyze immediately (within 2 hours). Or aliquot and store at -20 °C for long term. Avoid multiple freeze-thaw cycles.
Tissue lysate, body fluids or cell culture supernatants: Centrifuge to remove precipitate, analyze immediately or aliquot and store at -20 °C .
Serum: Coagulate the serum at room temperature (about 4 hours). Centrifuge at approximately 2000 × g for 20 min. Analyze the serum immediately or aliquot and store at -70 .° C Note: 1. Bovine serum used in cell culture supernates may contain abundant TGFȕ1, avoiding using it. 2. Coagulate blood samples completely, then, centrifuge, and avoid hemolysis and particle. 3. NaN3 can not be used as test sample preservative, since it is the inhibitor for HRP. >> Activate the sample ( if want to analyze the active form)
Tissue lysate, body fluids or cell culture supernate: add activating reagent pro rata, i.e. add 20ȝl of Solution A into 100ȝl of sample, 10 min later, add 20ȝl of Solution B. PH 7.0-7.6.
Serum: add activating reagent pro rata, i.e. add 20ȝl of Solution A into 40ȝl of sample, 10 min later, add 20ȝl of Solution B. PH 7.0-7.6.
It is unnecessary to activate the recombinant TGFȕ1.
Sample was diluted partly after adding activating reagent, so please pay attention to this when calculate target protein concentration.

Restrictions: For Research Use only

Stockage

Agent conservateur: Sodium azide, Thimerosal (Merthiolate)

Détail du antigène

Antigène: TGFB1 (Transforming Growth Factor, beta 1 (TGFB1))

Autre désignation: TGFbeta1

Sujet: Transforming growth factor beta 1 or TGFbeta1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. In humans, TGFbeta1 is encoded by the TGFB1 gene. This gene contains 7 exons and very large introns, maps to 19q13.1-q13.3. TGFbeta1 acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. The TGFB1 is directly involved in the pathogenesis of bone marrow reticulin fibrosis in hairy cell leukemia. The expression of TGFB1 in the early stages of DMD may be critical in initiating muscle fibrosis, and suggested that antifibrosis treatment might slow progression of the disease, increasing the utility of gene therapy.

Pathways: EGFR Signaling Pathway, Dopaminergic Neurogenesis, Cellular Response to Molecule of Bacterial Origin, Glycosaminoglycan Metabolic Process, Regulation of Leukocyte Mediated Immunity, Regulation of Muscle Cell Differentiation, Positive Regulation of Immune Effector Process, Cell-Cell Junction Organization, Production of Molecular Mediator of Immune Response, Ribonucleoside Biosynthetic Process, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy, Cancer Immune Checkpoints