FASL Kit ELISA

N° du produit ABIN1672725

Détail du produit FASL Kit ELISA

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Épitope: AA 134-281

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human FASL

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Analytical Method: Quantitative

Specificité: Expression system for standard: CHO
Immunogen sequence: P134-L281

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: <2pg/mL

Matériel non inclus: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogène: Expression system for standard: CHO
Immunogen sequence: P134-L281

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Plaque: Pre-coated

Protocole: human FASL ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for FASL has been precoated onto 96-well plates. Standards(CHO, P134-L281) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for FASL is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human FASL amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human FASL standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA, citrate) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human FASL standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 108, Standard deviation: 5.62, CV(%): 5.2
• Sample 2: n=16, Mean(pg/ml): 257, Standard deviation: 12.59, CV(%): 4.9
• Sample 3: n=16, Mean(pg/ml): 619, Standard deviation: 36.52, CV(%): 5.9,
• Sample 1: n=24, Mean(pg/ml): 112, Standard deviation: 9.74, CV(%): 8.7
• Sample 2: n=24, Mean(pg/ml): 249, Standard deviation: 22.66, CV(%): 9.1
• Sample 3: n=24, Mean(pg/ml): 623, Standard deviation: 44.86, CV(%): 7.2

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour FASL Kit ELISA (ABIN1672725)

Xu, Liu, Wu, Gong, Zhou, Qiao: "Proapoptotic effect of metalloproteinase 9 secreted by trophoblasts on endothelial cells." dans: The journal of obstetrics and gynaecology research, Vol. 37, Issue 3, pp. 187-94, (2011) (PubMed).

Xiong, Lu, Zhao, Xu, Bao, Lin, Yang: "Therapy with FasL-gene-modified dendritic cells confers a protective microenvironment in murine pregnancy." dans: Fertility and sterility, Vol. 93, Issue 8, pp. 2767-9, (2010) (PubMed).

Li, Peng, Li, Liu, Liang, Zhang: "Clinical significance of Fas and FasL protein expression in gastric carcinoma and local lymph node tissues." dans: World journal of gastroenterology, Vol. 16, Issue 10, pp. 1274-8, (2010) (PubMed).

Han, Qiu, Zhang, Kong, Wang, Wang, Li, Duan, Wang, Song, Wang: "Transplantation of sertoli-islet cell aggregates formed by microgravity: prolonged survival in diabetic rats." dans: Experimental biology and medicine (Maywood, N.J.), Vol. 234, Issue 5, pp. 595-603, (2009) (PubMed).

Détail du antigène

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Autre désignation: FASLG (FASL Produits)

Synonymes: ALPS1B Kit ELISA, APT1LG1 Kit ELISA, APTL Kit ELISA, CD178 Kit ELISA, CD95-L Kit ELISA, CD95L Kit ELISA, FASL Kit ELISA, TNFSF6 Kit ELISA, fasl Kit ELISA, Fas-L Kit ELISA, Faslg Kit ELISA, Tnfsf6 Kit ELISA, gld Kit ELISA, Apt1Lg1 Kit ELISA, Fasl Kit ELISA, FASLG Kit ELISA, zgc:162027 Kit ELISA, Fas ligand Kit ELISA, Fas ligand L homeolog Kit ELISA, Fas ligand (TNF superfamily, member 6) Kit ELISA, FASLG Kit ELISA, faslg.L Kit ELISA, Fasl Kit ELISA, Faslg Kit ELISA, faslg Kit ELISA

Sujet:

Background: Fas ligand(FasL or CD95L) is a type-II transmembrane protein that belongs to the tumor necrosis factor(TNF) family. Its binding with its receptor induces apoptosis. The human FASL gene consists of approximately 8 kb and is split into 4 exons. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Fas ligand or FasL is a homotrimeric type II transmembrane protein. It signals through trimerization of FasR, which spans the membrane of the "target" cell. This trimerization usually leads to apoptosis, or cell death. Soluble Fas ligand is generated by cleaving membrane-bound FasL at a conserved cleavage site by the external matrix metalloproteinase MMP-7.

Synonyms: FasL isoform ,Fasl ,

Full Gene Name: Fas ligand

ID gène: 14103

UniProt: Q99PH8

Pathways: Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity