Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

HBEGF Kit ELISA

HBEGF Reactivité: Humain AA 63-148 Colorimetric Sandwich ELISA 15.6-1000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1672797
  • Antigène Voir toutes HBEGF Kits ELISA
    HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
    Épitope
    AA 63-148
    Reactivité
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    15.6-1000 pg/mL
    Seuil minimal de détection
    15.6 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human HBEGF
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: sf21
    Immunogen sequence: D63-L148
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: sf21
    Immunogen sequence: D63-L148
    Featured
    Discover our best selling HBEGF Kit ELISA
    Top Product
    Discover our top product HBEGF Kit ELISA
  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Contains 1 EGF-like domain.

    Plaque
    Pre-coated
    Protocole
    human HBEGF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for HBEGF has been precoated onto 96-well plates. Standards(sf21, D63-L148) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for HBEGF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human HBEGF amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human HBEGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human HBEGF standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(pg/ml): 92.7, Standard deviation: 5.10, CV(%): 5.5
    • Sample 2: n=16, Mean(pg/ml): 369, Standard deviation: 16.97, CV(%): 4.6
    • Sample 3: n=16, Mean(pg/ml): 621, Standard deviation: 23.6, CV(%): 3.8,
    • Sample 1: n=24, Mean(pg/ml): 102, Standard deviation: 8.26, CV(%): 8.1
    • Sample 2: n=24, Mean(pg/ml): 376, Standard deviation: 27.82, CV(%): 7.4
    • Sample 3: n=24, Mean(pg/ml): 618, Standard deviation: 42.02, CV(%): 6.8
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes HBEGF Kits ELISA
    HBEGF (Heparin-Binding EGF-Like Growth Factor (HBEGF))
    Autre désignation
    HBEGF (HBEGF Produits)
    Synonymes
    hbegf Kit ELISA, zgc:165443 Kit ELISA, HBEGF Kit ELISA, zgc:175091 Kit ELISA, DTR Kit ELISA, DTS Kit ELISA, DTSF Kit ELISA, HEGFL Kit ELISA, Dtr Kit ELISA, GFHB Kit ELISA, Hb-egf Kit ELISA, Hegfl Kit ELISA, AW047313 Kit ELISA, Dts Kit ELISA, HB-EGF Kit ELISA, heparin-binding EGF-like growth factor b Kit ELISA, heparin binding EGF like growth factor Kit ELISA, Heparin-binding EGF-like growth factor Kit ELISA, heparin-binding EGF-like growth factor a Kit ELISA, heparin-binding EGF-like growth factor Kit ELISA, hbegfb Kit ELISA, HBEGF Kit ELISA, hbegf Kit ELISA, hbegfa Kit ELISA, Hbegf Kit ELISA
    Sujet

    Protein Function: Growth factor that mediates its effects via EGFR, ERBB2 and ERBB4. Required for normal cardiac valve formation and normal heart function. Promotes smooth muscle cell proliferation. May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts, but not endothelial cells. It is able to bind EGF receptor/EGFR with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

    Background: HBEGF(Heparin-binding EGF-like growth factor), also known as HEGFL or DTR, is a member of the EGF family of proteins that in humans is encoded by the HBEGF gene. The HBEGF gene is assigned to chromosome 5, thus confirming the assignment of the gene on the basis of its role in relation to diphtheria toxin susceptibility. HB-EGF is an 87 amino acid glycoprotein which displays highly regulated gene expression. It has been shown to play a role in wound healing, cardiac hypertrophy and heart development and function. HB-EGF binding and activation of EGF receptors plays a critical role during cardiac valve tissue development and the maintenance of normal heart function in adults.

    Synonyms: Proheparin-binding EGF-like growth factor,Heparin-binding EGF-like growth factor,HB-EGF,HBEGF,Diphtheria toxin receptor,DT-R,HBEGF,DTR, DTS, HEGFL,

    Full Gene Name: Proheparin-binding EGF-like growth factor

    Cellular Localisation: Heparin-binding EGF-like growth factor: Secreted, extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
    ID gène
    1839
    UniProt
    Q99075
    Pathways
    Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Vous êtes ici:
Support technique