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MME Kit ELISA

MME Reactivité: Souris AA 52-750 Colorimetric Sandwich ELISA 93.7-6000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1672816
  • Antigène Voir toutes MME Kits ELISA
    MME (Membrane Metallo-Endopeptidase (MME))
    Épitope
    AA 52-750
    Reactivité
    • 4
    • 3
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    93.7-6000 pg/mL
    Seuil minimal de détection
    93.7 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Neprilysin/CD10
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: sf21
    Immunogen sequence: Y52-W750
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: sf21
    Immunogen sequence: Y52-W750
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Plaque
    Pre-coated
    Protocole
    mouse CD10 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for CD10 has been precoated onto 96-well plates. Standards(sf21,Y52-W750) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD10 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse CD10 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 6000pg/mL, 3000pg/mL, 1500pg/mL, 750pg/mL, 375pg/mL, 187.5pg/mL, 93.7pg/m mouse CD10 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse CD10 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 0.59, Standard deviation: 0.037, CV(%): 6.3
    • Sample 2: n=16, Mean(ng/ml): 1.25, Standard deviation: 0.071, CV(%): 5.7
    • Sample 3: n=16, Mean(ng/ml): 3.79, Standard deviation: 0.197, CV(%): 5.2,
    • Sample 1: n=24, Mean(ng/ml): 0.53, Standard deviation: 0.046, CV(%): 8.7
    • Sample 2: n=24, Mean(ng/ml): 1.13, Standard deviation: 0.094, CV(%): 8.3
    • Sample 3: n=24, Mean(ng/ml): 3.85, Standard deviation: 0.277, CV(%): 7.2
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes MME Kits ELISA
    MME (Membrane Metallo-Endopeptidase (MME))
    Autre désignation
    MME (MME Produits)
    Synonymes
    CALLA Kit ELISA, CD10 Kit ELISA, NEP Kit ELISA, SFE Kit ELISA, 6030454K05Rik Kit ELISA, C85356 Kit ELISA, Nep Kit ELISA, neprilysin Kit ELISA, membrane metalloendopeptidase Kit ELISA, membrane metallo endopeptidase Kit ELISA, membrane metallo-endopeptidase Kit ELISA, MME Kit ELISA, Mme Kit ELISA
    Classe de substances
    Chemical
    Sujet

    Protein Function: Thermolysin-like specificity, but is almost confined on acting on polypeptides of up to 30 amino acids. Biologically important in the destruction of opioid peptides such as Met- and Leu-enkephalins by cleavage of a Gly-Phe bond. Able to cleave angiotensin-1, angiotensin-2 and angiotensin 1-9. Involved in the degradation of atrial natriuretic factor (ANF) (By similarity). Displays UV-inducible elastase activity toward skin preelastic and elastic fibers (PubMed:20876573). .

    Background: Neprilysin, also known as membrane metallo-endopeptidase, neutral endopeptidase(NEP), CD10, and common acute lymphoblastic leukemia antigen(CALLA), is a zinc-dependent metalloprotease enzyme that degrades a number of small secreted peptides, most notably theamyloid beta peptide whose abnormal misfolding and aggregation in neural tissue has been implicated as a cause of Alzheimer's disease, this gene is localized to human chromosome 3 by study of somatic cell hybrids and regionalized the location to 3q21-q27 by in situ hybridization. By cDNA transfection analysis, Neprilysin is confirmed as a functional neutral endopeptidase of the type that has previously been called enkephalinase. Neprilysin has also been called atriopeptidase. Atriopeptidase specifically degrades atrial natriuretic factor. A specific enzyme inhibitor was developed and reported that it had effects similar to those of low-dose ANF infusion. These effects include diuresis, natriuresis, vasodilatation, and suppression of the renin-angiotensin-aldosterone system.

    Synonyms: Neprilysin,3.4.24.11 ,Atriopeptidase,Enkephalinase,Neutral endopeptidase 24.11,NEP,Neutral endopeptidase,Skin fibroblast elastase,SFE,CD10,Mme,

    Full Gene Name: Neprilysin

    Cellular Localisation: Cell membrane, Single-pass type II membrane protein.
    ID gène
    17380
    UniProt
    Q61391
    Pathways
    Signalisation RTK, Peptide Hormone Metabolism, Regulation of Systemic Arterial Blood Pressure by Hormones, Smooth Muscle Cell Migration
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