Clusterin Kit ELISA

N° du produit ABIN1672832

Ce kit ELISA Colorimetric est destiné à la mesure quantitative de Humain Clusterin. Il a été cité dans 3 publications.

Aperçu rapide pour Clusterin Kit ELISA (ABIN1672832)

Antigène: Clusterin (CLU)

Épitope: AA 23-227, AA 228-449

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.78 ng/mL - 50 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Saliva, Urine

Détail du produit Clusterin Kit ELISA

Seuil minimal de détection: 0.78 ng/mL

Fonction: Sandwich Human Clusterin ELISA Kit to quantitate Human CLU in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva and urine.

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: D23-R227 (beta) & S228-E449 (alpha)

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 20 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 6.1%, 5.8%, 4.2%
Inter-Assay Precision (CV%): 6.3%, 6.3%, 5%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour Clusterin Kit ELISA (ABIN1672832)

Kim, Song, Kim, Jeon, Kim, Kang, Chun, Hong, Chung, Kim, Kim, Kim: "Urine clusterin/apolipoprotein J is linked to tubular damage and renal outcomes in patients with type 2 diabetes mellitus." dans: Clinical endocrinology, Vol. 87, Issue 2, pp. 156-164, (2018) (PubMed).

Song, Zhang, Zhang, Du, Guo, Kuang, Wang, Wu, Zou, Zou, Lv, Wang: "A microfluidic device for studying chemotaxis mechanism of bacterial cancer targeting." dans: Scientific reports, Vol. 8, Issue 1, pp. 6394, (2018) (PubMed).

Zheng, Yao, Qian, Sai, Qiu, Yang, Wu, Dong, Yao: "Oncogenic secretory clusterin in hepatocellular carcinoma: Expression at early staging and emerging molecular target." dans: Oncotarget, Vol. 8, Issue 32, pp. 52321-52332, (2017) (PubMed).

Détail du antigène

Antigène: Clusterin (CLU)

Autre désignation: CLU

Sujet:

Background: Clusterin (apolipoprotein J) is a 75 - 80 kDa disulfide-linked heterodimeric protein associated with the clearance of cellular debris and apoptosis.[1] In humans, clusterin is encoded by the CLU gene.This protein has several Synonyms: dimeric acidic glycoprotein (DAG protein), testosterone repressed prostate message-2 (TRPM-2), sulfated glycoprotein-2 (SGP-2) and complement lysis inhibitor (CLI). Clusterin was mapped to mouse chromosome 14. Clusterin is a ubiquitously expressed molecule thought to influence a variety of processes including cell death. In the brain, it accumulates in dying neurons following seizures and hypoxic-ischemic (H-I) injury. clusterin may be a new therapeutic target to modulate non-caspase-dependent neuronal death following acute brain injury.

Gene Full Name: clusterin

ID gène: 1191

UniProt: P10909

Pathways: Apoptose, Negative Regulation of intrinsic apoptotic Signaling