FASL Kit ELISA

N° du produit ABIN1672899

Le kit ELISA FASL (ABIN1672899) Rat est un kit ELISA Colorimetric conçu pour quantifier Rat FASL. Ce kit ELISA a été cité dans 2 publications.

Aperçu rapide pour FASL Kit ELISA (ABIN1672899)

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Épitope: AA 127-278

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Détail du produit FASL Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: Sandwich Rat FASL ELISA Kit to quantitate Rat Faslg in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Analytical Method: Quantitative

Specificité: Expression system for standard: NS0
Immunogen sequence: Q127-L278

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 6%, 4.2%, 4.3%
Inter-Assay Precision (CV%): 5.2%, 4.9%, 6%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour FASL Kit ELISA (ABIN1672899)

Jiang, Li, Zhou, Wang, Zhang, Wang: "Colistin-induced apoptosis in PC12 cells: involvement of the mitochondrial apoptotic and death receptor pathways." dans: International journal of molecular medicine, Vol. 33, Issue 5, pp. 1298-304, (2014) (PubMed).

Xu, Liu, Wu, Gong, Zhou, Qiao: "Proapoptotic effect of metalloproteinase 9 secreted by trophoblasts on endothelial cells." dans: The journal of obstetrics and gynaecology research, Vol. 37, Issue 3, pp. 187-94, (2011) (PubMed).

Détail du antigène

Antigène: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Autre désignation: FASLG

Sujet:

Background: FAS Ligand (FASL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. The human FasL gene consists of approximately 8.0 kb and is split into four exons. This gene consists of 281 amino acids with a calculated M (r) of 31,759 and was mapped on chromosome 1q23. It has an identity of 76.9 % at the amino acid sequence level with mouse FasL. The FAS and FASL system plays a key role in regulating apoptotic cell death and corruption of this signalling pathway has been shown to participate in immune escape and tumorigenesis. FAS and FASL triggered apoptosis pathway plays an important role in human carcinogenesis. This system may also play a role in modulating the genetic susceptibility of mouse strains to develop T-cell lymphoblastic lymphomas.

Gene Full Name: Fas ligand

ID gène: 25385

UniProt: P36940

Pathways: Apoptose, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity