KIT Ligand Kit ELISA

N° du produit ABIN1889301

Le kit ELISA KIT Ligand (ABIN1889301) Souris est un kit ELISA Colorimetric conçu pour quantifier Souris KIT Ligand. Ce kit ELISA a été cité dans 1 publication.

Aperçu rapide pour KIT Ligand Kit ELISA (ABIN1889301)

Antigène: KIT Ligand (KITLG)

Épitope: AA 26-190

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (EDTA)

Détail du produit KIT Ligand Kit ELISA

Seuil minimal de détection: 31.2 pg/mL

Fonction: Sandwich Mouse SCF ELISA Kit to quantitate Mouse Kitlg in cell culture supernatants, serum and plasma (EDTA).

Analytical Method: Quantitative

Specificité: Expression system for standard: E.coli
Immunogen sequence: K26-A190

Capture antibody and Detection antibody: monoclonal antibody from rat, polyclonal antibody from goat

Réactivité croisée (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Matériel non inclus: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Information d'application

Indications d'application: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Précision du teste: Intra-Assay Precision (CV%): 7%, 5.4%, 5.2%
Inter-Assay Precision (CV%): 8.6%, 6.7%, 5.3%

Restrictions: For Research Use only

Stockage

Conseil sur la manipulation: Avoid multiple freeze-thaw cycles.

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Date de péremption: 12 months

Références pour KIT Ligand Kit ELISA (ABIN1889301)

Barroeta Seijas, Simonetti, Vitale, Runci, Quinci, Soriani, Criscuoli, Filippi, Naldini, Sacchetti, Tarantino, Oliva, Piccirilli, Santoni, Di Rosa: "GM-CSF Inhibits c-Kit and SCF Expression by Bone Marrow-Derived Dendritic Cells." dans: Frontiers in immunology, Vol. 8, pp. 147, (2017) (PubMed).

Détail du antigène

Antigène: KIT Ligand (KITLG)

Autre désignation: KITLG

Sujet:

Background: Stem Cell Factor (also known as SCF, kit-ligand, KL, or steel factor) is a cytokine that binds to the c-Kit receptor (CD117). It is mapped to 12q21.32. SCF was primarily expressed by perivascular cells throughout the bone marrow, and it plays a role in the regulation of HSCs in the stem cell niche in the bone marrow. SCF can exist both as a transmembrane protein and a soluble protein. This gene plays an important role in the hematopoiesis during embryonic development. During development, the presence of the SCF also plays an important role in the localization of melanocytes, cells that produce melanin and control pigmentation. In addition to it, SCF can promote mast cell adhesion, migration, proliferation, and survival. It also promotes the release of histamine and tryptase, which are involved in the allergic response.

Gene Full Name: kit ligand

ID gène: 17311

UniProt: P20826

Pathways: Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway