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IGFBP5 Kit ELISA

IGFBP5 Reactivité: Souris AA 20-271 Colorimetric Sandwich ELISA 156-10.000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
N° du produit ABIN1889356
  • Antigène Voir toutes IGFBP5 Kits ELISA
    IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))
    Épitope
    AA 20-271
    Reactivité
    • 7
    • 6
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156-10.000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    Fonction
    Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse IGFBP5
    Marque
    PicoKine™
    Type d'échantillon
    Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)
    Analytical Method
    Quantitative
    Specificité
    Expression system for standard: NSO
    Immunogen sequence: L20-E271
    Réactivité croisée (Details)
    There is no detectable cross-reactivity with other relevant proteins.
    Sensibilité
    <10pg/mL
    Matériel non inclus
    Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl
    Immunogène
    Expression system for standard: NSO
    Immunogen sequence: L20-E271
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  • Indications d'application
    Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.
    Commentaires

    Sequence similarities: Contains 1 IGFBP N-terminal domain.

    Tissue Specificity: Most abundant in kidney, uterus and gastrocnemius muscle.

    Plaque
    Pre-coated
    Protocole
    mouse IGFBP5 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for IGFBP5 has been precoated onto 96-well plates. Standards(NSO, L20-E271) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGFBP5 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse IGFBP5 amount of sample captured in plate.
    Procédure de l'essai

    Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse IGFBP5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse IGFBP5 standard solution and each sample be measured in duplicate.

    Précision du teste
    • Sample 1: n=16, Mean(ng/ml): 1.1, Standard deviation: 0.045, CV(%): 4.1
    • Sample 2: n=16, Mean(ng/ml): 3.4, Standard deviation: 0.167, CV(%): 4.9
    • Sample 3: n=16, Mean(ng/ml): 6.2, Standard deviation: 0.341, CV(%): 5.5,
    • Sample 1: n=24, Mean(ng/ml): 1.5, Standard deviation: 0.084, CV(%): 5.6
    • Sample 2: n=24, Mean(ng/ml): 3.8, Standard deviation: 0.247, CV(%): 6.5
    • Sample 3: n=24, Mean(ng/ml): 6.7, Standard deviation: 0.476, CV(%): 7.1
    Restrictions
    For Research Use only
  • Conseil sur la manipulation
    Avoid multiple freeze-thaw cycles.
    Stock
    -20 °C,4 °C
    Stockage commentaire
    Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles
    Date de péremption
    12 months
  • Antigène Voir toutes IGFBP5 Kits ELISA
    IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))
    Autre désignation
    IGFBP5 (IGFBP5 Produits)
    Synonymes
    IBP5 Kit ELISA, AI256729 Kit ELISA, AW208790 Kit ELISA, IGFBP-5 Kit ELISA, IGFBP-5P Kit ELISA, IGF-BP5 Kit ELISA, igfbp5 Kit ELISA, ibp5 Kit ELISA, IBP-5 Kit ELISA, xIGFBP-5 Kit ELISA, IGFBP5 Kit ELISA, igfbp5b Kit ELISA, zgc:165472 Kit ELISA, insulin like growth factor binding protein 5 Kit ELISA, insulin-like growth factor binding protein 5 Kit ELISA, insulin like growth factor binding protein 5 L homeolog Kit ELISA, insulin-like growth factor binding protein 5b Kit ELISA, insulin-like growth factor-binding protein 5 Kit ELISA, insulin-like growth factor binding protein 5a Kit ELISA, IGFBP5 Kit ELISA, Igfbp5 Kit ELISA, igfbp5.L Kit ELISA, igfbp5b Kit ELISA, igfbp5 Kit ELISA, LOC100194500 Kit ELISA, igfbp5a Kit ELISA
    Sujet

    Protein Function: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors.

    Background: Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The IGFBP5 gene was mapped to chromosome 2q33-q34. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection was inhibitory to growth in 2 human breast cancer cell lines. Stable expression of IGFBP5 in the breast cancer cell lines also inhibited the formation and growth of tumors following injection in athymic mice. IGFBP5 is believed a growth inhibitor and proapoptotic agent in breast cancer cells.

    Synonyms: Insulin-like growth factor-binding protein 5,IBP-5,IGF-binding protein 5,IGFBP-5,Igfbp5,Igfbp-5,

    Full Gene Name: Insulin-like growth factor-binding protein 5

    Cellular Localisation: Secreted.
    ID gène
    16011
    UniProt
    Q07079
    Pathways
    Signalisation WNT, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding
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