CAMK2B Kit ELISA
Aperçu rapide pour CAMK2B Kit ELISA (ABIN2043049)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 0.31 ng/mL
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Sensibilité
- < 0.113 ng/mL
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Plaque
- Pre-coated
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Protocole
- Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))
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Autre désignation
- Calcium/Calmodulin Dependent Protein Kinase II beta (CAMK2b)
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Pathways
- Signalisation WNT, Interferon-gamma Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity
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