C3 Kit ELISA

N° du produit ABIN2043197

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Poulet C3.

Aperçu rapide pour C3 Kit ELISA (ABIN2043197)

Antigène: C3 (Complement Component 3 (C3))

Reactivité: Poulet

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.25-2000 ng/mL

Application: ELISA

Détail du produit C3 Kit ELISA

Seuil minimal de détection: 31.25 ng/mL

Sensibilité: < 11.2 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Complement Component 3 (C3) BioAssay-ELISA Kit (Chicken) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 3 (C3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 3 (C3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 3 (C3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Complement Component 3 (C3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: C3 (Complement Component 3 (C3))

Pathways: Système du Complément, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Regulation of G-Protein Coupled Receptor Protein Signaling