EGLN1 Kit ELISA

N° du produit ABIN2043387

Ce kit ELISA Souris EGLN1 est un kit ELISA Colorimetric conçu pour quantifier Souris EGLN1.

Aperçu rapide pour EGLN1 Kit ELISA (ABIN2043387)

Antigène: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit EGLN1 Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Sensibilité: < 0.108 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Egl Nine Homolog 1 (EGLN1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Egl Nine Homolog 1 (EGLN1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Egl Nine Homolog 1 (EGLN1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Egl Nine Homolog 1 (EGLN1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Egl Nine Homolog 1 (EGLN1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: EGLN1 (Egl-9 Family Hypoxia Inducible Factor 1 (EGLN1))

Autre désignation: Egl Nine Homolog 1 (EGLN1)

Pathways: cAMP Metabolic Process, L'effet Warburg