FAK Kit ELISA

N° du produit ABIN2043497

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Rat FAK.

Aperçu rapide pour FAK Kit ELISA (ABIN2043497)

Antigène: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit FAK Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Sensibilité: < 0.112 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Focal Adhesion Kinase (FAK) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Focal Adhesion Kinase (FAK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Focal Adhesion Kinase (FAK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Focal Adhesion Kinase (FAK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Focal Adhesion Kinase (FAK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: FAK (PTK2) (PTK2 Protein tyrosine Kinase 2 (PTK2))

Autre désignation: Focal Adhesion Kinase (FAK)

Pathways: Response to Growth Hormone Stimulus, CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling