GADD45A Kit ELISA

N° du produit ABIN2043599

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Rat GADD45A.

Aperçu rapide pour GADD45A Kit ELISA (ABIN2043599)

Antigène: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit GADD45A Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.062 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Growth Arrest And DNA Damage Inducible Protein Alpha (GADD45a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: GADD45A (Growth Arrest and DNA-Damage-Inducible, alpha (GADD45A))

Autre désignation: Growth Arrest And DNA Damage Inducible Protein alpha (GADD45a)

Pathways: Signalisation p53, Cycle Cellulaire