IGF1R Kit ELISA
Aperçu rapide pour IGF1R Kit ELISA (ABIN2043753)
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Méthode de détection
Type de méthode
Gamme de detection
Application
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Seuil minimal de détection
- 0.15 ng/mL
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Sensibilité
- < 0.057 ng/mL
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Plaque
- Pre-coated
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Protocole
- Insulin Like Growth Factor 1 Receptor (IGF1R) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Insulin Like Growth Factor 1 Receptor (IGF1R). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Insulin Like Growth Factor 1 Receptor (IGF1R). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Insulin Like Growth Factor 1 Receptor (IGF1R), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Insulin Like Growth Factor 1 Receptor (IGF1R) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Stock
- 4 °C/-20 °C
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- IGF1R (Insulin-Like Growth Factor 1 Receptor (IGF1R))
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Autre désignation
- Insulin Like Growth Factor 1 Receptor (IGF1R)
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Pathways
- Signalisation RTK, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Autophagy
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