HLA-A Kit ELISA

N° du produit ABIN2043913

Kit ELISA Humain HLA-A, test Colorimetric pour la quantification de Humain HLA-A.

Aperçu rapide pour HLA-A Kit ELISA (ABIN2043913)

Antigène: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.31-20 ng/mL

Application: ELISA

Détail du produit HLA-A Kit ELISA

Seuil minimal de détection: 0.31 ng/mL

Sensibilité: < 0.126 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: Major Histocompatibility Complex Class I A (MHCA) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Major Histocompatibility Complex Class I A (MHCA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Major Histocompatibility Complex Class I A (MHCA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Major Histocompatibility Complex Class I A (MHCA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Major Histocompatibility Complex Class I A (MHCA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: HLA-A (Major Histocompatibility Complex, Class I, A (HLA-A))

Autre désignation: Major Histocompatibility Complex Class I A (MHCA)

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints, Human Leukocyte Antigen (HLA) in Adaptive Immune Response