NRF2 Kit ELISA

N° du produit ABIN2044123

Kit ELISA Rat NRF2, test Colorimetric pour la quantification de Rat NRF2.

Aperçu rapide pour NRF2 Kit ELISA (ABIN2044123)

Antigène: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62-1000 pg/mL

Application: ELISA

Détail du produit NRF2 Kit ELISA

Seuil minimal de détection: 15.62 pg/mL

Sensibilité: < 5.5 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: NRF2 (NFE2L2) (Nuclear Factor (erythroid-Derived 2)-Like 2 (NFE2L2))

Autre désignation: Nuclear Factor, Erythroid Derived 2 Like Protein 2 (NFE2L2)

Pathways: ER-Nucleus Signaling, Negative Regulation of intrinsic apoptotic Signaling