WIF1 Kit ELISA

N° du produit ABIN2044847

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain WIF1.

Aperçu rapide pour WIF1 Kit ELISA (ABIN2044847)

Antigène: WIF1 (WNT Inhibitory Factor 1 (WIF1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.62-1000 pg/mL

Application: ELISA

Détail du produit WIF1 Kit ELISA

Seuil minimal de détection: 15.62 pg/mL

Sensibilité: < 6.9 pg/mL

Information d'application

Plaque: Pre-coated

Protocole: WNT Inhibitory Factor 1 (WIF1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to WNT Inhibitory Factor 1 (WIF1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to WNT Inhibitory Factor 1 (WIF1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain WNT Inhibitory Factor 1 (WIF1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of WNT Inhibitory Factor 1 (WIF1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: WIF1 (WNT Inhibitory Factor 1 (WIF1))

Pathways: Signalisation WNT, Positive Regulation of fat Cell Differentiation