XBP1 Kit ELISA

N° du produit ABIN2044857

Le kit ELISA XBP1 (ABIN2044857) Rat est un kit ELISA Colorimetric conçu pour quantifier Rat XBP1.

Aperçu rapide pour XBP1 Kit ELISA (ABIN2044857)

Antigène: XBP1 (X-Box Binding Protein 1 (XBP1))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-10 ng/mL

Application: ELISA

Détail du produit XBP1 Kit ELISA

Seuil minimal de détection: 0.15 ng/mL

Sensibilité: < 0.054 ng/mL

Information d'application

Plaque: Pre-coated

Protocole: X-Box Binding Protein 1 (XBP1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to X-Box Binding Protein 1 (XBP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to X-Box Binding Protein 1 (XBP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain X-Box Binding Protein 1 (XBP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of X-Box Binding Protein 1 (XBP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Stockage

Stock: 4 °C/-20 °C

Détail du antigène

Antigène: XBP1 (X-Box Binding Protein 1 (XBP1))

Pathways: ER-Nucleus Signaling, Unfolded Protein Response