IL-13 Kit ELISA

N° du produit ABIN2111993

Ce kit ELISA Colorimetric est conçu pour la mesure quantitative de Humain IL-13.

Aperçu rapide pour IL-13 Kit ELISA (ABIN2111993)

Antigène: IL-13 (IL13) (Interleukin 13 (IL13))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6-1000 pg/mL

Application: ELISA

Détail du produit IL-13 Kit ELISA

Seuil minimal de détection: 15.6 pg/mL

Analytical Method: Quantitative

Sensibilité: < 2 pg/mL

Ingrédients: L-13 MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
IL-13 STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTION

Information d'application

Plaque: Pre-coated

Protocole: This assay applies the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific to IL-13 has been pre-coated onto a microplate. Standards and samples are then added to the appropriate plate wells with a biotin-conjugated antibody preparation specific for IL-13 and incubated. IL-13 if present, will bind and become immobilized by the antibody pre-coated on the wells and then be "sandwiched" by biotin conjugate. Avidin is a tetramer containing four identical subunits that each has a high affinity-binding site for biotin. After washing away any unbound substances, avidin-Horseradish Peroxidase (HRP) will be added to each well and incubated. Following a wash to remove any unbound antibody-enzyme reagent, a substrate solution is added to the wells and only those wells that contain IL-13, biotin conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The color development is stopped and the intensity of the color is measured.

Restrictions: For Research Use only

Stockage

Détail du antigène

Antigène: IL-13 (IL13) (Interleukin 13 (IL13))

Autre désignation: Interleukin-13

Sujet: IL-13 is also associated with many features of the allergic airway diseases, including mucus hypersecretion, goblet metaplasia, chemokine induction and recruitment of effector cells. All of them contribute to airway obstruction. Polymorphisms in IL-13 gene have been found to link to increased eosinophil count, serum total IgE and high risk of asthma.

Pathways: Signalistation JAK/STAT, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Proton Transport