CCL2 Kit ELISA
-
- Antigène Voir toutes CCL2 Kits ELISA
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
-
Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Competition ELISA
- Gamme de detection
- 7.8-500 pg/mL
- Seuil minimal de détection
- 7.8 pg/mL
- Application
- ELISA
- Marque
- BioAssay™
- Sensibilité
- < 1.7 pg/mL
- Ingrédients
-
MCP-1 MICROTITER PLATE
BIOTIN CONJUGATE
AVIDIN CONJUGATE
MCP-1 STANDARD
CALIBRATOR DILUENT I
CALIBRATOR DILUENT II
WASH BUFFER (20X/96 wells, 30X/192 wells)
SUBSTRATE A
SUBSTRATE B
STOP SOLUTION - Featured
- Discover our best selling CCL2 Kit ELISA
- Top Product
- Discover our top product CCL2 Kit ELISA
-
-
- Plaque
- Pre-coated
- Protocole
- Elevated MCP-1 levels are associated with autoimmune disease, allergic inflammation, atherosclerosis, glomerulonephritis, granuloma formation. This ELISA kit provides a tool for studying MCP-1 expression and its relationship with various diseases in animal model.This mouse MCP-1 enzyme-linked immunosorbent assay (ELISA) applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for mouse MCP-1. Standards or samples are then added to the appropriate microtiter plate wells and incubated. Mouse MCP-1, if present, will bind and become immobilized by the antibody pre-coated on the wells. A biotin conjugated antibody specific for mouse MCP-1 is added to each well. The biotin conjugated antibody will bind to the mouse MCP-1 on the plate. The microtiter plate wells are thoroughly washed to remove unbound biotin conjugate and other components of the sample. Avidin has a very high affinity to biotin. In order to quantify the amount of mouse MCP-1 present in the sample, a standardized preparation of avidin conjugated horseradish peroxidase (HRP) is added to each well. Avidin-HRPwill bind to the biotin on plate during incubation. The wells are thoroughly washed to remove all unbound Avidin-HRPconjugate and aTMB(3,3'5,5' tetramethyl-benzidine) substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only wells to which mouse MCP-1, Biotin conjugate and Avidin-HRPare attached will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 2nm.
- Restrictions
- For Research Use only
-
- Stock
- 4 °C/-20 °C
-
- Antigène Voir toutes CCL2 Kits ELISA
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
- Autre désignation
- MCP-1 (CCL2 Produits)
- Synonymes
- GDCF-2 Kit ELISA, HC11 Kit ELISA, HSMCR30 Kit ELISA, MCAF Kit ELISA, MCP-1 Kit ELISA, MCP1 Kit ELISA, SCYA2 Kit ELISA, SMC-CF Kit ELISA, AI323594 Kit ELISA, JE Kit ELISA, Scya2 Kit ELISA, Sigje Kit ELISA, MCP-1A Kit ELISA, MCP1A Kit ELISA, C-C motif chemokine ligand 2 Kit ELISA, chemokine (C-C motif) ligand 2 Kit ELISA, C-C motif chemokine 2 Kit ELISA, CCL2 Kit ELISA, Ccl2 Kit ELISA, LOC101120093 Kit ELISA
- Pathways
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, Phosphorylation & l'infection par le SRAS-CoV-2
-