CD86 Kit ELISA

N° du produit ABIN2648551

Kit ELISA Humain CD86, test Colorimetric pour la quantification de Humain CD86.

Aperçu rapide pour CD86 Kit ELISA (ABIN2648551)

Antigène: CD86

Reactivité: Humain

Méthode de détection: Colorimetric

Application: ELISA

Type d'échantillon: Serum, Plasma, Cell Culture Cells

Détail du produit CD86 Kit ELISA

Fonction: Human CD86 ELISA is to be used for the in-vitro quantitative determination CD86 in human serum, plasma, buffered solutions or cell culture medium. The assay will recognize both natural and recombinant human CD86.

Analytical Method: Quantitative

Sensibilité: 0.6 U/ml

Attributs du produit: Human CD86 ELISA Assay kit is for Research Use Only.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 2 - 3 h

Plaque: Pre-coated

Protocole: The CD86 Kit is a solid phase sandwich Enzyme Linked-Immuno- Sorbent Assay (ELISA). A monoclonal antibody specific for CD86 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known CD86 concentrations and unknowns are pipetted into these wells. During the first incubation, the CD86 antigen and a biotinylated monoclonal antibody specific for CD86 are simultaneously incubated. After washing, the enzyme (streptavidin-peroxydase) is added. After incubation and washing to remove all the unbound enzyme, a substrate solution is added to induce a coloured reaction product. The intensity of this coloured product is directly proportional to the concentration of CD86 present in the samples.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: CD86

Autre désignation: CD86

Pathways: TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation