Interferon gamma Kit ELISA

N° du produit ABIN2648682

Ce kit ELISA Souris Interferon gamma est un kit ELISA Colorimetric conçu pour quantifier Souris Interferon gamma.

Aperçu rapide pour Interferon gamma Kit ELISA (ABIN2648682)

Antigène: Interferon gamma (IFNG)

Reactivité: Souris

Méthode de détection: Colorimetric

Application: ELISA

Type d'échantillon: Serum, Plasma, Cell Culture Cells

Détail du produit Interferon gamma Kit ELISA

Fonction: Mouse Interferon Gamma (IFN-γ) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of mouse Interferon Gamma (IFN-γ) concentrations in cell culture supernates, serum, and plasma.

Analytical Method: Quantitative

Sensibilité: 7 pg/ml

Attributs du produit: Mouse Interferon Gamma (IFN-γ) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures.

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Volume d'échantillon: 100 μL

Durée du test: 3 h

Plaque: Pre-coated

Protocole: Mouse Interferon Gamma (IFN-γ) ELISA Assay Kit the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for IFN-γ has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any IFN-γ present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for IFN-γ is added to the wells and binds to the combination of capture antibody-IFN-γ in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of IFN-γ present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven IFN-γ standard dilutions and IFN-γ sample concentration determined.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Détail du antigène

Antigène: Interferon gamma (IFNG)

Autre désignation: IFN-gamma

Pathways: Interferon-gamma Pathway, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Protein targeting to Nucleus, Autophagy