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ADAMTS1 Kit ELISA

ADAMTS1 Reactivité: Humain, Souris Colorimetric Sandwich ELISA 0.4-100 ng/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2702804
  • Antigène Voir toutes ADAMTS1 Kits ELISA
    ADAMTS1 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 1 (ADAMTS1))
    Reactivité
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain, Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.4-100 ng/mL
    Seuil minimal de détection
    0.4 ng/mL
    Application
    ELISA
    Fonction
    Mouse ADAMTS-1 (METH1) ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair detects mouse and human ADAMTS1. Other species not determined.
    Sensibilité
    0.4 ng/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Top Product
    Discover our top product ADAMTS1 Kit ELISA
  • Indications d'application
    Recommended Dilution for serum and plasma samples2 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes ADAMTS1 Kits ELISA
    ADAMTS1 (ADAM Metallopeptidase with Thrombospondin Type 1 Motif, 1 (ADAMTS1))
    Autre désignation
    ADAMTS-1 (ADAMTS1 Produits)
    Synonymes
    c3-c5 Kit ELISA, meth1 Kit ELISA, xadamts1 Kit ELISA, C3-C5 Kit ELISA, METH1 Kit ELISA, ADAM-TS1 Kit ELISA, ADAMTS Kit ELISA, ADAMTS-1 Kit ELISA, METH-1 Kit ELISA, adamts1 Kit ELISA, ADAM metallopeptidase with thrombospondin type 1 motif 1 Kit ELISA, ADAM metallopeptidase with thrombospondin type 1 motif, 1 Kit ELISA, a disintegrin-like and metallopeptidase (reprolysin type) with thrombospondin type 1 motif, 1 Kit ELISA, ADAM metallopeptidase with thrombospondin type 1 motif 1 L homeolog Kit ELISA, ADAMTS1 Kit ELISA, adamts1 Kit ELISA, Adamts1 Kit ELISA, adamts1.L Kit ELISA
    Sujet
    Gene Names: Adamts1
    Protein names: A disintegrin and metalloproteinase with thrombospondin motifs 1 (ADAM-TS 1) (ADAM-TS1) (ADAMTS-1) (EC 3.4.24.-)
    ID gène
    11504
    UniProt
    P97857
    Pathways
    SARS-CoV-2 Protein Interactome
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