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FGF5 Kit ELISA

FGF5 Reactivité: Humain Colorimetric Sandwich ELISA 24-6.000 pg/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN2703015
  • Antigène Voir toutes FGF5 Kits ELISA
    FGF5 (Fibroblast Growth Factor 5 (FGF5))
    Reactivité
    • 3
    • 2
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    24-6.000 pg/mL
    Seuil minimal de détection
    24 pg/mL
    Application
    ELISA
    Fonction
    Human FGF-5 ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Cell Culture Supernatant, Plasma, Serum
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair detects human FGF-5. Other species not determined.
    Sensibilité
    24 pg/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Indications d'application
    Recommended Dilution for serum and plasma samples2 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes FGF5 Kits ELISA
    FGF5 (Fibroblast Growth Factor 5 (FGF5))
    Autre désignation
    FGF-5 (FGF5 Produits)
    Synonymes
    FGF5 Kit ELISA, HBGF-5 Kit ELISA, Smag-82 Kit ELISA, Fgf-5 Kit ELISA, angora Kit ELISA, go Kit ELISA, FGF-5 Kit ELISA, fibroblast growth factor 5 Kit ELISA, FGF5 Kit ELISA, fgf5 Kit ELISA, Fgf5 Kit ELISA
    Sujet
    Gene Names: FGF5
    Protein names: Fibroblast growth factor 5 (FGF-5) (Heparin-binding growth factor 5) (HBGF-5) (Smag-82)
    ID gène
    2250
    UniProt
    P12034
    Pathways
    Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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