Kallikrein 6 Kit ELISA

N° du produit ABIN2703283

Détail du produit Kallikrein 6 Kit ELISA

Antigène: Kallikrein 6 (KLK6)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.6-150 ng/mL

Seuil minimal de détection: 0.6 ng/mL

Application: ELISA

Fonction: Human Kallikrein 6 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detects human KLK6. Other species not determined.

Sensibilité: 0.6 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour Kallikrein 6 Kit ELISA (ABIN2703283)

Ehrenfeld, Manso, Pavicic, Matus, Borquez, Lizama, Sarmiento, Poblete, Bhoola, Naran, Figueroa: "Bioregulation of kallikrein-related peptidases 6, 10 and 11 by the kinin B₁ receptor in breast cancer cells." dans: Anticancer research, Vol. 34, Issue 12, pp. 6925-38, (2015) (PubMed).

Détail du antigène

Antigène: Kallikrein 6 (KLK6)

Autre désignation: KLK6 (KLK6 Produits)

Synonymes: Bssp Kit ELISA, Klk7 Kit ELISA, PRSS18 Kit ELISA, PRSS9 Kit ELISA, SP59 Kit ELISA, hK6 Kit ELISA, Prss9 Kit ELISA, AI849898 Kit ELISA, BSP Kit ELISA, Klk29 Kit ELISA, MSP Kit ELISA, Prss18 Kit ELISA, neurosin Kit ELISA, kallikrein related peptidase 6 Kit ELISA, kallikrein related-peptidase 6 Kit ELISA, kallikrein-6 Kit ELISA, KLK6 Kit ELISA, Klk6 Kit ELISA, LOC100716801 Kit ELISA

Sujet: Gene Names: KLK6 PRSS18 PRSS9
Protein names: Kallikrein-6 (EC 3.4.21.-) (Neurosin) (Protease M) (SP59) (Serine protease 18) (Serine protease 9) (Zyme)

ID gène: 5653

UniProt: Q92876

Pathways: Système du Complément, Regulation of G-Protein Coupled Receptor Protein Signaling