FLT1 Kit ELISA

N° du produit ABIN2703569

Kit ELISA Humain FLT1, test Colorimetric pour la quantification de Humain FLT1 et a été mentionné dans 1 publication.

Aperçu rapide pour FLT1 Kit ELISA (ABIN2703569)

Antigène: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 1.5-400 ng/mL

Application: ELISA

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Détail du produit FLT1 Kit ELISA

Seuil minimal de détection: 1.5 ng/mL

Fonction: Human VEGFR1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Analytical Method: Quantitative

Specificité: Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human Activin A, AgRP, Angiogenin, ANG-1, Angiostatin, Cathepsin S, CD40, Cripto-1, DAN, DKK-1, E-Cadherin, EpCAM, Fas L, Follistatin, Galectin-7, ICAM-2, IL-13 R1, IL-13R2, IL-17B, IL-2R alpha, IL-2 R beta, IL-23, LAP, NrCAM, PAI-1, PDGF-AB, Resistin, SDF-1 beta, sgp130, Shh N, Siglec-5, ST2, TGF-beta 2, Tie-2, TPO, TRAIL R4, TREM-1, VEGF-C.

Sensibilité: 1.5 ng/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples2 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Références pour FLT1 Kit ELISA (ABIN2703569)

Wood, Cox, Phelps, Lai, Poddar, Talbot, Mu: "Thyroid Transcription Factor 1 Reprograms Angiogenic Activities of Secretome." dans: Scientific reports, Vol. 6, pp. 19857, (2016) (PubMed).

Détail du antigène

Antigène: FLT1 (Fms-Related tyrosine Kinase 1 (VEGFR1) (FLT1))

Autre désignation: VEGFR1

Sujet: Gene Names: FLT1 FLT FRT VEGFR1
Protein names: Vascular endothelial growth factor receptor 1 (VEGFR-1) (EC 2.7.10.1) (Fms-like tyrosine kinase 1) (FLT-1) (Tyrosine-protein kinase FRT) (Tyrosine-protein kinase receptor FLT) (FLT) (Vascular permeability factor receptor)

ID gène: 2321

UniProt: P17948

Pathways: Signalisation RTK, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals