Growth Hormone 1 Kit ELISA
Aperçu rapide pour Growth Hormone 1 Kit ELISA (ABIN2708231)
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Méthode de détection
Type de méthode
Gamme de detection
Application
Type d'échantillon
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Seuil minimal de détection
- 156 pg/mL
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Fonction
- Sandwich ELISA for quantitative detection of Human GH1 concentrations.
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Analytical Method
- Quantitative
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Sensibilité
- 59 pg/mL
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Ingrédients
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- Coated 96-well Plate
- Standard
- Biotin-Labeled Detection Antibody (100X)
- Streptavidin-HRP (100X)
- Standard/Sample Diluent
- Detection Antibody Diluent
- Streptavidin-HRP Diluent
- Wash Buffer (20X)
- TMB Substrate Solution
- Stop Solution
- Plate Adhesive Strips
- Technical Manual
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Matériel non inclus
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- Microplate reader capable of measuring absorbance at 450 nm.
- Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
- Adjustable 1-25 ml pipettes for reagent preparation.
- 100 ml and 1 liter graduated cylinders.
- Absorbent paper.
- Distilled or deionized water.
- Computer and software for ELISA data analysis.
- Tubes to prepare standard or sample dilutions.
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Growth Hormone 1 (GH1) ELISA Kit
GH1 Reactivité: Poulet Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Growth Hormone 1 (GH1) ELISA KitGH1 Reactivité: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 Reactivité: Porc Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum
Growth Hormone 1 (GH1) ELISA KitGH1 Reactivité: Chien Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum
Growth Hormone 1 (GH1) ELISA KitGH1 Reactivité: Boeuf (Vache) Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 Reactivité: Souris Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 Reactivité: Chien Colorimetric Sandwich ELISA 0.625 ng/mL - 40 ng/mL Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 Reactivité: Poulet Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 Reactivité: Poulet Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Growth Hormone 1 (GH1) ELISA KitGH1 Reactivité: Boeuf (Vache) Colorimetric Competition ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
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Indications d'application
- Optimal working dilution should be determined by the investigator.
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Volume d'échantillon
- 100 μL
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Plaque
- Pre-coated
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Protocole
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- Prepare all reagents, samples and standards
- Add 100 µL Standard or Sample
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Biotin-Labeled Detection Antibody Working Solution
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Steptavidin-HRP Working Solution
- Wash plate 5 times with Wash Buffer Working Solution
- Add 100 µL TMB Substrat Solution
- Add 100 µL Stop Solution
- Read the plate at 450 nm
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Restrictions
- For Research Use only
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Conseil sur la manipulation
- Avoid freeze/thaw cycles
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Stock
- 4 °C,-20 °C
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Stockage commentaire
- Shipped and store at 4°C for 6 months, store at -20°C for one year.
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Date de péremption
- 6-12 months
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- Growth Hormone 1 (GH1)
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Autre désignation
- GH1
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Sujet
- Alternative Names: Somatotropin, Growth hormone, GH, GH-N, Growth hormone 1, Pituitary growth hormone
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ID gène
- 2688
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UniProt
- P01241
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Pathways
- Signalisation NF-kappaB, Signalistation JAK/STAT, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Response to Growth Hormone Stimulus, Regulation of Hormone Biosynthetic Process
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