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Adipsin Kit ELISA

CFD Reactivité: Macaque rhésus Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN2747937
  • Antigène Voir toutes Adipsin (CFD) Kits ELISA
    Adipsin (CFD) (Complement Factor D (CFD))
    Reactivité
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    Macaque rhésus
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Rhesus Macaque Adipsin (CFD) ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    The antibody pair provided in this kit recognizes Rhesus Macaque Adipsin (CFD)
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Procédure de l'essai

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes Adipsin (CFD) Kits ELISA
    Adipsin (CFD) (Complement Factor D (CFD))
    Autre désignation
    Adipsin (CFD Produits)
    Synonymes
    ADIPSIN Kit ELISA, ADN Kit ELISA, DF Kit ELISA, PFD Kit ELISA, Adn Kit ELISA, Df Kit ELISA, EVE Kit ELISA, CFD Kit ELISA, adipsin Kit ELISA, pfd Kit ELISA, cfdl Kit ELISA, wu:fb61f12 Kit ELISA, zgc:109940 Kit ELISA, 10.5 Kit ELISA, 10.9 Kit ELISA, 14.10 Kit ELISA, 20.35 Kit ELISA, CG2328 Kit ELISA, Dmel\\CG2328 Kit ELISA, E(eve) Kit ELISA, Eve Kit ELISA, F Kit ELISA, V Kit ELISA, VI Kit ELISA, eve2 Kit ELISA, even Kit ELISA, l(2)46CFg Kit ELISA, l(2)46CFh Kit ELISA, l(2)46CFj Kit ELISA, l(2)46CFp Kit ELISA, l(2)46Ce Kit ELISA, l(2)46Cg Kit ELISA, complement factor D Kit ELISA, complement factor D (adipsin) Kit ELISA, complement factor D (adipsin) L homeolog Kit ELISA, even skipped Kit ELISA, CFD Kit ELISA, Cfd Kit ELISA, cfd.L Kit ELISA, cfd Kit ELISA, eve Kit ELISA
    Sujet
    Adipsin (CFD)
    UniProt
    F6YBP7
    Pathways
    Système du Complément
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