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MAP2K6 Kit ELISA

MAP2K6 Reactivité: Humain, Souris, Rat pSer207 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN2748365
  • Antigène Voir toutes MAP2K6 Kits ELISA
    MAP2K6 (Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6))
    Épitope
    pSer207
    Reactivité
    • 5
    • 4
    • 2
    • 1
    Humain, Souris, Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human/Mouse/Rat Phospho-MKK6 (S207) ELISA Kit. This assay semi-quantitatively measures phophorylated MKK6 (Ser207) in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes human, mouse, and rat MKK6 phosphorylated at site Serine-207
    Attributs du produit
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Top Product
    Discover our top product MAP2K6 Kit ELISA
  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Procédure de l'essai

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes MAP2K6 Kits ELISA
    MAP2K6 (Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6))
    Autre désignation
    MKK6 (MAP2K6 Produits)
    Synonymes
    MAPKK6 Kit ELISA, MEK6 Kit ELISA, MKK6 Kit ELISA, PRKMK6 Kit ELISA, SAPKK-3 Kit ELISA, SAPKK3 Kit ELISA, Mkk6 Kit ELISA, Prkmk6 Kit ELISA, MKK3 Kit ELISA, map2k3 Kit ELISA, zMKK3 Kit ELISA, map2k6 Kit ELISA, mitogen-activated protein kinase kinase 6 Kit ELISA, mitogen-activated protein kinase kinase 6 L homeolog Kit ELISA, MAP2K6 Kit ELISA, Map2k6 Kit ELISA, map2k6 Kit ELISA, map2k6.L Kit ELISA
    Sujet
    Dual specificity mitogen-activated protein kinase kinase 6, MAP kinase kinase 6 (MAPKK 6), MAPK/ERK kinase 6 phosphorylated at Serine-207
    ID gène
    5608
    UniProt
    P52564
    Pathways
    Signalisation MAPK, Signalisation TLR, Activation of Innate immune Response, Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades
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