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CDKN1B Kit ELISA

CDKN1B Reactivité: Rat Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
N° du produit ABIN2748476
  • Antigène Voir toutes CDKN1B Kits ELISA
    CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
    Reactivité
    • 7
    • 5
    • 3
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Custom Rat p27/Kip1 (CDKN1B) ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    The antibody pair provided in this kit recognizes Rat p27/Kip1 (CDKN1B)
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Procédure de l'essai

    Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes CDKN1B Kits ELISA
    CDKN1B (Cyclin-Dependent Kinase Inhibitor 1B (p27, Kip1) (CDKN1B))
    Autre désignation
    p27/Kip1 (CDKN1B Produits)
    Synonymes
    CDKN4 Kit ELISA, KIP1 Kit ELISA, MEN1B Kit ELISA, MEN4 Kit ELISA, P27KIP1 Kit ELISA, cdkn1b Kit ELISA, sb:cb611 Kit ELISA, wu:fb63g10 Kit ELISA, wu:fb64g10 Kit ELISA, wu:fe18e03 Kit ELISA, CDKN1B Kit ELISA, Kip1 Kit ELISA, p27 Kit ELISA, FAM14D Kit ELISA, ISG12 Kit ELISA, ISG12A Kit ELISA, P27 Kit ELISA, Cdki1b Kit ELISA, AA408329 Kit ELISA, AI843786 Kit ELISA, p27Kip1 Kit ELISA, cdkn1bl Kit ELISA, cyclin dependent kinase inhibitor 1B Kit ELISA, cyclin-dependent kinase inhibitor 1Bb Kit ELISA, interferon alpha inducible protein 27 Kit ELISA, cyclin-dependent kinase inhibitor 1B Kit ELISA, cyclin-dependent kinase inhibitor 1Ba Kit ELISA, CDKN1B Kit ELISA, cdkn1bb Kit ELISA, IFI27 Kit ELISA, Cdkn1b Kit ELISA, cdkn1ba Kit ELISA
    Sujet
    P27/Kip1 (CDKN1B)
    ID gène
    83571
    UniProt
    O08769
    Pathways
    Cycle Cellulaire, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Peptide Hormone Secretion, Negative Regulation of Hormone Secretion, Sensory Perception of Sound, Mitotic G1-G1/S Phases, DNA Replication, Positive Regulation of Endopeptidase Activity, Synthesis of DNA, Autophagy
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