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RPS6KA1 Kit ELISA

RPS6KA1 Reactivité: Humain pSer380 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
N° du produit ABIN2748547
  • Antigène Voir toutes RPS6KA1 Kits ELISA
    RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))
    Épitope
    pSer380
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Phosph-RSK1 (S380) ELISA Kit. This assay semi-quantitatively measures phophorylated RSK1 (Ser380) in lysate samples.
    Type d'échantillon
    Cell Lysate, Tissue Lysate
    Analytical Method
    Semi-Quantitative
    Specificité
    The antibody pair provided in this kit recognizes human RSK1 phosphorylated at site Serine-380 .
    Attributs du produit
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Matériel non inclus
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Procédure de l'essai

    Prepare all reagents and samples as instructed in the manual.
    Add 100 μL of sample or positive control to each well.
    Incubate 2.5 h at RT or O/N at 4 °C.
    Add 100 μL of prepared primary antibody to each well.
    Incubate 1 h at RT.
    Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    Incubate 1 h at RT.
    Add 100 μL of TMB One-Step Substrate Reagent to each well.
    Incubate 30 min at RT.
    Add 50 μL of Stop Solution to each well.
    Read at 450 nm immediately.

    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Date de péremption
    6 months
  • Antigène Voir toutes RPS6KA1 Kits ELISA
    RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))
    Autre désignation
    RSK1 (RPS6KA1 Produits)
    Synonymes
    HU-1 Kit ELISA, MAPKAPK1A Kit ELISA, RSK Kit ELISA, RSK1 Kit ELISA, Rsk1 Kit ELISA, p90rsk Kit ELISA, rsk Kit ELISA, RPS6KA Kit ELISA, hu-1 Kit ELISA, mapkapk1a Kit ELISA, rsk1 Kit ELISA, wu:fc56h07 Kit ELISA, zgc:152654 Kit ELISA, ribosomal protein S6 kinase A1 Kit ELISA, ribosomal protein S6 kinase polypeptide 1 Kit ELISA, ribosomal protein S6 kinase A1-like Kit ELISA, ribosomal protein S6 kinase A1 L homeolog Kit ELISA, ribosomal protein S6 kinase a, polypeptide 1 Kit ELISA, RPS6KA1 Kit ELISA, Rps6ka1 Kit ELISA, RPS6KA1L Kit ELISA, rps6ka1.L Kit ELISA, rps6ka1 Kit ELISA
    Sujet
    Phospho-RSK1 (S380)
    ID gène
    6195
    UniProt
    Q15418
    Pathways
    Signalisation MAPK, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades
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