IGF1 Kit ELISA

N° du produit ABIN2859206

Détail du produit IGF1 Kit ELISA

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Épitope: AA 49-118

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5-4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: For quantitative detection of human IGF-1 in cell culture supernates, serum and plasma(heparin, EDTA).

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Analytical Method: Quantitative

Specificité: Natural and recombinant human IGF-1

Réactivité croisée (Details): There is no detectable cross-reactivity with IGF-2.

Sensibilité: <10pg/mL

Ingrédients:

• 96-well plate precoated with anti- human IGF-1 antibod - 1
• Lyophilized recombinant human IGF-1 standar - 10ng/tubex2
• Biotinylated anti- human IGF-1 antibod - 130μl(dilution 1:100)
• Avidin-Biotin-Peroxidase Complex (ABC - 130μl(dilution 1:100)
• Sample diluent buffe - 30 ml
• Antibody diluent buffe - 12ml
• ABC diluent buffe - 12ml
• TMB color developing agen - 10ml
• TMB stop solutio - 10ml

Immunogène: Expression system for standard: E.coli
Immunogen sequence: G49-A118

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole: human IGF-1 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for IGF-1 has been precoated onto 96-well plates. Standards(E.coli, G49-A118) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for IGF-1 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human IGF-1 amount of sample captured in plate.

Procédure de l'essai:

Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human IGF-1 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human IGF-1 standard solution and each sample be measured in duplicate.

Précision du teste:

• Sample 1: n=16, Mean(pg/ml): 523, Standard deviation: 21.97, CV(%): 4.2
• Sample 2: n=16, Mean(pg/ml): 1345, Standard deviation: 65.91, CV(%): 4.9
• Sample 3: n=16, Mean(pg/ml): 2632, Standard deviation: 134.2, CV(%): 5.1,
• Sample 1: n=24, Mean(pg/ml): 645, Standard deviation: 37.41, CV(%): 5.8
• Sample 2: n=24, Mean(pg/ml): 1631, Standard deviation: 109.3, CV(%): 6.7
• Sample 3: n=24, Mean(pg/ml): 3045, Standard deviation: 231.4, CV(%): 7.6

Restrictions: For Research Use only

Stockage

Stock: -20 °C,4 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Date de péremption: 12 months

Références pour IGF1 Kit ELISA (ABIN2859206)

Wang, Wang, Liang, Liu, Shi, Bai, Lin, Magaye, Zhao: "Expression and clinical significance of IGF-1, IGFBP-3, and IGFBP-7 in serum and lung cancer tissues from patients with non-small cell lung cancer." dans: OncoTargets and therapy, Vol. 6, pp. 1437-44, (2013) (PubMed).

Cai, Li, Wang, Liu, Yang, Chen, Yin, Tan, Zhu, Pan, Wang, Lu: "Apoptosis of bone marrow mesenchymal stem cells caused by homocysteine via activating JNK signal." dans: PLoS ONE, Vol. 8, Issue 5, pp. e63561, (2013) (PubMed).

Ni, Sun, Fu, Wang, Guo, Tian, Wei: "IGF-1 promotes the development and cytotoxic activity of human NK cells." dans: Nature communications, Vol. 4, pp. 1479, (2013) (PubMed).

Chai, Guo, Wang, Fu, Guan, Tan, Ren, Yang: "Antibacterial effect of 317L stainless steel contained copper in prevention of implant-related infection in vitro and in vivo." dans: Journal of materials science. Materials in medicine, Vol. 22, Issue 11, pp. 2525-35, (2011) (PubMed).

Détail du antigène

Antigène: IGF1 (Insulin-Like Growth Factor 1 (IGF1))

Autre désignation: IGF1 (IGF1 Produits)

Synonymes: IGF-I Kit ELISA, IGF1A Kit ELISA, IGFI Kit ELISA, C730016P09Rik Kit ELISA, Igf-1 Kit ELISA, Igf-I Kit ELISA, Npt2B Kit ELISA, IGF-1 Kit ELISA, IGFIA Kit ELISA, IGF-IB Kit ELISA, igf1 Kit ELISA, IGF-1L Kit ELISA, IGF-1a Kit ELISA, IGF1 Kit ELISA, igf-1 Kit ELISA, igf1-A Kit ELISA, igf1.S Kit ELISA, xigf1 Kit ELISA, insulin like growth factor 1 Kit ELISA, insulin-like growth factor 1 Kit ELISA, insulin-like growth factor I Kit ELISA, insulin like growth factor 1 L homeolog Kit ELISA, IGF1 Kit ELISA, Igf1 Kit ELISA, LOC100136741 Kit ELISA, igf1 Kit ELISA, LOC678666 Kit ELISA, igf1.L Kit ELISA, LOC104911603 Kit ELISA

Sujet: Insulin-like growth factor 1(IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD.1 The IGF1 gene, mapped on 12q22-q24. contains 5 exons. Exons 1-4 encode the 195-amino acid precursor(IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide(IGF1A). The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98 % of IGF-1 is always bound to one of 6 binding proteins(IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.

ID gène: 3479

UniProt: P05019

Pathways: Signalisation RTK, Intracellular Steroid Hormone Receptor Signaling Pathway, Peptide Hormone Metabolism, Hormone Activity, Regulation of Intracellular Steroid Hormone Receptor Signaling, Regulation of Hormone Metabolic Process, Regulation of Hormone Biosynthetic Process, Stem Cell Maintenance, Glycosaminoglycan Metabolic Process, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Activated T Cell Proliferation, Positive Regulation of fat Cell Differentiation